The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes
The effect of natural extracellular matrix deposited on synthetic polymers on cultured primary hepatocytes
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DOI：
10.1016/j.biomaterials.2006.04.014
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发表时间：
2006-09-01
期刊：
影响因子：
14
通讯作者：
Akaike, Toshihiro
中科院分区：
文献类型：
--
作者：
Hoshiba, Takashi;Cho, Chong Su;Akaike, Toshihiro
众所周知，在细胞培养过程中，天然细胞外基质（ECM）分子沉积在生物材料的表面并影响细胞的行为。然而，尽管沉积的ECM影响细胞的行为，但在生物材料表面沉积的ECM分子种类并不完全清楚。本研究旨在探讨天然细胞外基质（ECM）对体外培养的肝细胞在生物材料（如聚乳酸、聚乳酸、聚乳酸等）上的行为的影响，作为肝细胞特异性基质的N-对乙烯基苄基-4-O-β-D-吡喃半乳糖基-D-甘露酰胺（PVLA）和聚（L-赖氨酸）（PLL）在体外肝细胞培养过程中作为非特异性的一种，我们研究了ECM基因的表达模式和ECM分子在PVLA和PLL包被表面上的吸附。结果发现，在PVLA包被的表面上培养的肝细胞中的I型胶原和纤维连接蛋白基因的表达水平与在PLL包被的表面上培养的肝细胞中的I型胶原和纤维连接蛋白基因的表达水平不同。此外，结果表明，层粘连蛋白主要沉积在PVLA涂层表面，而纤连蛋白主要沉积在PLL涂层表面。肝细胞在PVLA和层粘连蛋白包被的表面上保持肝脏特异性功能。据认为，在肝细胞培养过程中沉积的层粘连蛋白影响在PVLA包被的表面上培养的肝细胞的肝脏特异性功能。(c)2006爱思唯尔有限公司版权所有。
It is well known that natural extracellular matrix (ECM) molecules are deposited on the surface of biomaterials during culture of cells and affect cellular behaviors. However, it has not been fully understood what kinds of ECM molecules are deposited on the surface of biomaterials although the cellular behaviors were affected by deposited ECM. In this study, to investigate the effect of deposited natural ECM on behaviors of hepatocytes cultured on biomaterials such as poly (N-p-vinylbenzyl-4-O-beta-D-galactopyranosyl-D-gluconamide) (PVLA) as a hepatocyte-specific matrix and poly (L-lysine) (PLL) as a non-specific one during the culture of hepatocytes in vitro, we investigated expression pattern of ECM genes and adsorption of ECM molecules onto PVLA- and PLL-coated surfaces. It was found that the expression levels of type I collagen and fibronectin genes in the hepatocytes cultured on PVLA-coated surface were different from them in the hepatocytes cultured on PLL-coated one. Also, the results showed that laminin was dominantly deposited on PVLA-coated surface whereas fibronectin was dominantly deposited on PLL-coated one. Hepatocytes maintained liver-specific functions on PVLA- and laminin-coated surfaces. It is thought that deposited laminin during the culture of hepatocytes affects the liver-specific functions of hepatocytes cultured on PVLA-coated surface. (c) 2006 Elsevier Ltd. All rights reserved.