Isolation of alpha-toxin, theta-toxin and kappa-toxin mutants of Clostridium perfringens by Tn916 mutagenesis
Isolation of alpha-toxin, theta-toxin and kappa-toxin mutants of Clostridium perfringens by Tn916 mutagenesis
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DOI：
10.1006/mpat.1996.0115
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发表时间：
1997-05-01
影响因子：
3.8
通讯作者：
Rood, JI
中科院分区：
文献类型：
--
作者：
Awad, MM;Rood, JI
产气荚膜梭菌是梭状芽胞杆菌肌肉坏死或气性坏疽的病原体，通过产生包括α-毒素、β-毒素和kappa-毒素在内的多种细胞外毒素而介导感染和疾病。Tn916-突变用于分离产生α-毒素或β-毒素能力有缺陷的突变体。共分离到9个独立来源的突变体。在其中4个突变体中，Tn916插入了Theta毒素结构基因pFOA上游193个或198个碱基的位置。四个突变体包含较大的缺失，其中三个位于分别包含theta毒素结构和调控基因pfOA和pfoR的区域，以及K-毒素结构基因Cola，以及一个包含a-毒素结构基因PIE的区域。这些突变体应该被证明对进一步的基因研究是非常有价值的，这些基因研究旨在确定这些毒素在毒力中的作用。(C)1997年学术出版社有限公司。
Clostridium perfringens is the causative agent of clostridial myonecrosis or gas gangrene and mediates infection and disease by producing numerous extracellular toxins, including alpha-toxin, theta-toxin and kappa-toxin. Tn916-mutagenesis was used to isolate mutants defective in their ability to produce either alpha-toxin or theta-toxin. Nine independently derived mutants were isolated. In four of these mutants Tn916 had inserted at sites located 193 bp or 198 bp upstream of the theta-toxin structural gene, pfoA. Four mutants contained large deletions, three in regions which encompassed the theta-toxin structural and regulatory genes pfoA and pfoR, respectively, and the K-toxin structural gene, colA, and one in a region encompassing the a-toxin structural gene, pie. These mutants should prove to be invaluable for further genetic studies aimed at determining the role of these toxins in virulence. (C) 1997 Academic Press Limited.