A homeobox gene, vax2, controls the patterning of the eye dorsoventral axis
A homeobox gene, vax2, controls the patterning of the eye dorsoventral axis
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DOI：
10.1073/pnas.96.19.10729
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发表时间：
1999-09-14
影响因子：
11.1
通讯作者：
Banfi, S
中科院分区：
文献类型：
--
作者：
Barbieri, AM;Lupo, G;Banfi, S
我们已经确定了一个转录因子，特别是在发展中的脊椎动物的眼睛表达。我们将该基因命名为vax 2，因为它与最近描述的vax 1具有高度的序列相似性。在人类和小鼠基因组中，vax 2都位于emx 1基因附近。这一映射分配，连同先前报道的Vax 1和Emx 2在小鼠中的共定位，表明var和emx基因可能被组织成簇。VAX 2具有局限于小鼠、人和非洲爪蟾中的预期神经视网膜的腹侧部分的显著表达结构域。在非洲爪蟾胚胎中青蛙XAX 2或人VAX 2的过表达导致异常的眼表型，并且特别地，决定了对发育中的眼的腹侧效应。转录因子Xpax 2的表达结构域通常局限于腹侧发育的视网膜，在vax 2过表达后延伸到视网膜的背侧区域。另一方面，背侧视网膜的分子标记物Xvent 2的表达强烈降低。此外，vax2过表达诱导视柄的显著扩张，视柄是源自眼囊的近腹区域的结构。总之，这些数据表明，vax2在眼睛发育中起着至关重要的作用，特别是在腹侧视泡的规格。
We have identified a transcription factor specifically expressed in the developing vertebrate eye. We named this gene vax2 because of the high degree of sequence similarity to the recently described vax1, Both in the human and mouse genomes, vax2 is localized in the vicinity of the emx1 gene. This mapping assignment, together with the previously reported colocalization of Vax1 and Emx2 in mouse, indicates that the var and the emx genes may be organized in clusters. vax2 has a remarkable expression domain confined to the ventral portion of the prospective neural retina in mouse, human, and Xenopus, The overexpression of either the frog Xvax2 or the human VAX2 in Xenopus embryos leads to an aberrant eye phenotype and, in particular, determines a ventralizing effect on the developing eye. The expression domain of the transcription factor Xpax2, normally confined to the ventral developing retina, extends to the dorsal region of the retina after overexpression of vax2, On the other hand, the expression of Xvent2, a molecular marker of the dorsal retina, is strongly reduced. Furthermore, vax2 overexpression induces a striking expansion of the optic stalk, a structure deriving from the ventralmost region of the eye vesicle. Altogether, these data indicate that vax2 plays a crucial role in eye development and, in particular, in the specification of the ventral optic vesicle.