Cordon-bleu is a conserved gene involved in neural tube formation
Cordon-bleu is a conserved gene involved in neural tube formation
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DOI：
10.1016/s0012-1606(03)00323-3
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发表时间：
2003-10-01
影响因子：
2.7
通讯作者：
Klingensmith, J
中科院分区：
文献类型：
--
作者：
Carroll, EA;Gerrelli, D;Klingensmith, J
在脊椎动物胚胎中，中轴线是图案和形态发生线索的重要来源。中线来源于原肠胚中的一小群细胞，被称为“组织者”，以表彰其组织整个身体计划的能力。哺乳动物的组织者，即节点，产生轴向中线结构：脊索，背侧前肠和神经管底板的一部分。只有一些指导中线发育的基因是已知的。在这项研究中，我们提出了一个新的基因，cordon-bleu（cobl）的完整编码序列，特异性地表达在节点及其衍生物，直到器官发生阶段。推导出的序列与任何已知功能的基因都不相似。然而，cobl是广泛保守的：在许多脊椎动物物种中发现了明显的直系同源物和旁系同源物，其中几个序列结构域高度保守，但功能未知。我们发现，鸡蓝带是类似的表达在节点及其衍生物，这表明功能的保护。我们还报告了相关小鼠基因Coblr 1的序列和非重叠表达。最后，我们表明，cobl与神经形成基因Vangl 2相互作用，以促进中脑神经管关闭，证明cobl和Vangl 2在中脑神经形成中的作用。(C)2003年爱思唯尔公司All rights reserved.
The axial midline is an important source of patterning and morphogenesis cues in the vertebrate embryo. The midline derives from a small group of cells in the gastrulating embryo, known as "the organizer" in recognition of its ability to organize an entire body plan. The mammalian organizer, the node, gives rise to axial midline structures: the notochord, dorsal foregut, and part of the floor plate of the neural tube. Only some of the genes that direct midline development are known. In this study, we present the complete coding sequence for a novel gene, cordon-bleu (cobl), expressed specifically in the node and its derivatives until organogenesis stages. The deduced sequence does not resemble any gene of known function. However, cobl is widely conserved: apparent orthologs and paralogs are found in many vertebrate species, with several sequence domains of high conservation but unknown function. We find that chicken cordon-bleu is similarly expressed in the node and its derivatives, suggesting functional conservation. We also report the sequence and nonoverlapping expression of a related mouse gene, Coblr1. Finally, we show that cobl interacts with the neurulation gene Vangl2 to facilitate midbrain neural tube closure, demonstrating roles for both cobl and Vangl2 in midbrain neurulation. (C) 2003 Elsevier Inc. All rights reserved.