Mosaic analysis of extended auricle1 (eta1) suggests that a two-way signaling pathway is involved in positioning the blade/sheath boundary in Zea mays.
Mosaic analysis of extended auricle1 (eta1) suggests that a two-way signaling pathway is involved in positioning the blade/sheath boundary in Zea mays.
复制标题
对延伸耳廓 1 (eta1) 的马赛克分析表明，双向信号通路参与了玉米叶片/鞘边界的定位。
DOI：
10.1016/j.ydbio.2005.11.012
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Freeling,Michael
中科院分区：
文献类型：
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作者：
Osmont,KarenS;Sadeghian,Nasim;Freeling,Michael
玉米叶片以一种简单的、典型的方式发育；因此，它是理解形成发育边界所涉及的过程的基本模型。扩展叶绿体1(Eta1)是一个影响近端叶片发育的多效性玉米突变体。突变型eta1个体表现出叶片/鞘边界的基瓣位移，耳廓和叶片之间的边界不清楚，导致耳廓起伏。扫描电子显微镜分析表明，Eta1是叶片/鞘边界在叶片近轴面正确放置所必需的。对维管和细胞组织的检测表明，eta1不仅影响叶片/鞘边界的位置，还影响叶片/鞘边界内细胞类型的分化。利用遗传镶嵌分析确定了eta1突变组织对野生型叶片发育的影响，并确定了eta1+基因产物的位置和时间。有趣的是，即使在野生型组织中，eta1组织的扇区也会影响刀片/鞘边界的位置。这些结果表明，叶片/鞘边界的定位可能涉及一条双向信号通路。基于这些数据，我们提出了玉米叶片中Eta1+的功能模型。
The maize leaf develops in a simple, stereotypical manner; therefore, it serves as a basic model to understand the processes involved in forming developmental boundaries. extended auricle1 (eta1) is a pleiotropic maize mutant that affects proximodistal leaf development. Mutant eta1 individuals display basipetal displacement of the blade/sheath boundary and the boundary between auricle and blade is not clearly delineated, leading to an undulating auricle. SEM analysis shows that eta1 is required for proper placement of the blade/sheath boundary on the adaxial leaf surface. Examination of vascular and cellular organization indicates that eta1 affects not only placement of the blade/sheath boundary, but also differentiation of cell types within the blade/sheath boundary. Genetic mosaic analysis was used to determine the effect of eta1 mutant tissue on wild-type leaf development and to resolve the site and timing of the Eta1+ gene product. Interestingly, sectors of eta1 tissue affect the placement of the blade/sheath boundary even in wild-type tissue. These results suggest that a two-way signaling pathway may be involved in the positioning of the blade/sheath boundary. Based on these data, we propose a model for Eta1+ function in the maize leaf.