RNA-seq analysis of broiler liver transcriptome reveals novel responses to high ambient temperature.
RNA-seq analysis of broiler liver transcriptome reveals novel responses to high ambient temperature.
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DOI：
10.1186/1471-2164-15-1084
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发表时间：
2014-12-10
期刊：
影响因子：
4.4
通讯作者：
Lamont SJ
中科院分区：
文献类型：
--
作者：
Coble DJ;Fleming D;Persia ME;Ashwell CM;Rothschild MF;Schmidt CJ;Lamont SJ
在肉鸡中，高环境温度可导致饲料消耗减少，消化效率低下，代谢受损，甚至死亡。美国家禽业的肉鸡部门每年遭受约5200万美元的热相关损失。本研究的目的是表征循环高环境温度对代谢活性器官肝脏转录组的影响。这项研究为高环境温度对肉鸡代谢的影响提供了新的见解，因为它是首次报道的RNA-seq研究，以表征热对代谢相关组织转录组的影响。这些信息为未来的研究提供了一个平台，以进一步阐明对高环境温度的生理反应，并寻求改善热的负面影响的方法。使用Illumina HiSeq 2000技术对8只雄性肉鸡的肝脏进行转录组测序，得到1.38亿个100个碱基对的单端读段，产生总共13.8千兆碱基的序列。结果表明，在一周的循环高温胁迫下，40个基因的表达差异达到显著水平（P < 0.05），其中27个基因表达下调，13个基因表达上调。从差异表达基因的基于功能的损伤途径分析（IPA）创建两个基因网络：“细胞信号传导”和“内分泌系统发育和功能”。热暴露肉鸡肝脏转录组中的基因表达差异反映了降低内部温度、减少高血压诱导的细胞凋亡和促进组织修复的生理反应。此外，差异基因表达揭示了调节高环境温度暴露可能导致的扰动细胞钙水平的生理反应。暴露于周期性高环境温度导致代谢、生理和细胞水平的变化，这些变化可以通过肉鸡肝脏转录组的RNA-seq分析来表征。研究结果强调了肉鸡减轻暴露于高环境温度影响的特定生理机制。这些信息为未来研究参与肉鸡应激反应的基因网络以及制定改善高温对动物生产和福利的负面影响的策略提供了基础。
In broilers, high ambient temperature can result in reduced feed consumption, digestive inefficiency, impaired metabolism, and even death. The broiler sector of the U.S. poultry industry incurs approximately $52 million in heat-related losses annually. The objective of this study is to characterize the effects of cyclic high ambient temperature on the transcriptome of a metabolically active organ, the liver. This study provides novel insight into the effects of high ambient temperature on metabolism in broilers, because it is the first reported RNA-seq study to characterize the effect of heat on the transcriptome of a metabolic-related tissue. This information provides a platform for future investigations to further elucidate physiologic responses to high ambient temperature and seek methods to ameliorate the negative impacts of heat. Transcriptome sequencing of the livers of 8 broiler males using Illumina HiSeq 2000 technology resulted in 138 million, 100-base pair single end reads, yielding a total of 13.8 gigabases of sequence. Forty genes were differentially expressed at a significance level of P-value < 0.05 and a fold-change ≥ 2 in response to a week of cyclic high ambient temperature with 27 down-regulated and 13 up-regulated genes. Two gene networks were created from the function-based Ingenuity Pathway Analysis (IPA) of the differentially expressed genes: “Cell Signaling” and “Endocrine System Development and Function”. The gene expression differences in the liver transcriptome of the heat-exposed broilers reflected physiological responses to decrease internal temperature, reduce hyperthermia-induced apoptosis, and promote tissue repair. Additionally, the differential gene expression revealed a physiological response to regulate the perturbed cellular calcium levels that can result from high ambient temperature exposure. Exposure to cyclic high ambient temperature results in changes at the metabolic, physiologic, and cellular level that can be characterized through RNA-seq analysis of the liver transcriptome of broilers. The findings highlight specific physiologic mechanisms by which broilers reduce the effects of exposure to high ambient temperature. This information provides a foundation for future investigations into the gene networks involved in the broiler stress response and for development of strategies to ameliorate the negative impacts of heat on animal production and welfare.
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影响因子：
3.7
作者：
Cha MY;Han SH;Son SM;Hong HS;Choi YJ;Byun J;Mook-Jung I
通讯作者：
Mook-Jung I
影响因子：
1.7
作者：
Billings SE;Clarke GL;Nishimune H
通讯作者：
Nishimune H
影响因子：
8
作者：
Adachi, K;Toyota, M;Tokino, T
通讯作者：
Tokino, T
DOI：
10.1046/j.1439-0396.2000.00223.x
发表时间：
2000-03-01
期刊：
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION-ZEITSCHRIFT FUR TIERPHYSIOLOGIE TIERERNAHRUNG UND FUTTERMITTELKUNDE
影响因子：
--
作者：
Hai, L;Rong, D;Zhang, ZY
通讯作者：
Zhang, ZY
影响因子：
--
作者：
Friesema, Edith C. H.;Kuiper, George G. J. M.;Kester, Monique H. A.
通讯作者：
Kester, Monique H. A.