VIBRIO-CHOLERAE SOLUBLE HEMAGGLUTININ PROTEASE IS A METALLOENZYME
VIBRIO-CHOLERAE SOLUBLE HEMAGGLUTININ PROTEASE IS A METALLOENZYME
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DOI：
10.1128/iai.42.2.639-644.1983
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
FINKELSTEIN, RA
中科院分区：
文献类型：
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作者：
BOOTH, BA;BOESMANFINKELSTEIN, M;FINKELSTEIN, RA
由霍乱弧菌产生的可溶性血凝素/蛋白酶，其先前已显示水解纤连蛋白和卵粘蛋白并切割乳铁蛋白和大肠杆菌的不耐热肠毒素的A亚基，似乎是锌金属内肽酶。它的血凝和蛋白酶功能都被螯合剂抑制，包括Zincov，一种被设计用于抑制锌金属蛋白酶的异羟肟酸衍生物。嗜热菌蛋白酶，一种已知的含锌蛋白酶，也会引起鸡红细胞的血凝反应。该活性被Zincov抑制，其不影响胰蛋白酶和链霉蛋白酶的血凝活性。血凝素/蛋白酶对呋喃丙烯酰-Gly-Leu-NH 2有活性，呋喃丙烯酰-Gly-Leu-NH 2是嗜热菌蛋白酶和其他类似蛋白酶的合成底物。感染霍乱弧菌或经霍乱毒素处理的幼兔肠液的血凝活性不受Zincov抑制，这表明该活性不是由于血凝素/蛋白酶所致，如先前所提出的。
A soluble hemagglutinin/protease produced by V. cholerae, which has previously been shown to hydrolyze fibronectin and ovomucin and to cleave lactoferrin and the A subunit of the heat-labile enterotoxin of Escherichia coli, appears to be a zinc metalloendopeptidase. Both its hemagglutinative and protease functions are inhibited by chelating agents, inlcuding Zincov, a hydroxamic acid derivative sepcifically designed to inhibit zinc metalloproteases. Thermolysin, a known Zn-containing protease, also causes hemagglutination of responder chicken erythrocytes. This activity is inhibited by Zincov, which does not affect the hemagglutination activity of trypsin and pronase. The hemagglutinin/protease is active on furylacryloyl-Gly-Leu-NH2, a synthetic substrate for thermolysin and other similar proteases. The hemagglutination activity of V. cholerae-infected or cholera toxin-treated infant rabbit intestinal fluid is not inhibited by Zincov, which suggests that this activity is not due to the hemagglutinin/protease, as formerly proposed.