Circulating miR-92a expression level in patients with essential hypertension: a potential marker of atherosclerosis
Circulating miR-92a expression level in patients with essential hypertension: a potential marker of atherosclerosis
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DOI：
10.1038/jhh.2016.66
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发表时间：
2017-03-01
影响因子：
2.7
通讯作者：
Feng, Y-q
中科院分区：
文献类型：
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作者：
Huang, Y.;Tang, S.;Feng, Y-q
MicroRNAs(MiRs)是所有真核细胞中基因表达的关键转录后调节因子，在高血压和心血管重塑的进化中起着至关重要的作用，因此已成为心血管疾病的潜在生物标志物。我们评估了240名受试者，包括60名颈动脉内膜中层厚度(NCIMT)正常的健康志愿者、60名CIMT增厚的健康志愿者、60名高血压伴nCIMT的患者和60名高血压伴iCIMT的患者。所有患者均进行CIMT、颈动脉-股动脉脉搏波速度(CfPWV)和动态血压(BP)监测。用实时定量逆转录聚合酶链式反应检测血浆miR-92a的表达。应用Spearman相关系数分析miR-92a表达与血压参数、CIMT和cfPWV的相关性。我们观察到miR-92a在nCIMT患者中的表达最低(24.59+/-1.30vs27.76+/-2.13vs29.29+/-1.89vs33.76+/-2.08；P<；0.001)，其次是iCIMT患者，然后是高血压患者并iCIMT患者。此外，MIR-92a水平与24小时平均收缩压(r=0.807，P<0.001)、24小时平均舒张压(r=0.649，P<0.001)、24小时平均脉压(r=0.697，P<0.001)、24小时白天脉压(r=0.654，P<0.001)、24小时夜间脉压(r=0.573，P<0.05)呈显著正相关。0.001)、CIMT(r=0.571，P<；0.001)和cfPWV(r=0.601，P<；0.001)。我们的数据提供了重要证据，表明循环miR-92a是高血压患者潜在的非侵入性动脉粥样硬化标志物。
MicroRNAs (miRs) are key posttranscriptional regulators of gene expression in all eukaryotic cells and have a vital role in the evolution of hypertension and cardiovascular remodelling and, therefore, have emerged as potential biomarkers for cardiovascular disease. We assessed 240 participants, including 60 healthy volunteers with normal carotid intima-media thickness (nCIMT), 60 healthy volunteers with increased CIMT (iCIMT), 60 hypertensive patients with nCIMT and 60 hypertensive patients with iCIMT. All patients underwent measurements of CIMT, carotid-femoral pulse wave velocity (cfPWV) and ambulatory blood pressure (BP) monitoring. Plasma miR-92a expression was quantified by real-time reverse transcription PCR. Correlations between miR-92a expression and BP parameters, CIMT and cfPWV were assessed using the Spearman correlation coefficient. We observed the lowest miR-92a expression (24.59 +/- 1.30 vs 27.76 +/- 2.13 vs 29.29 +/- 1.89 vs 33.76 +/- 2.08; P < 0.001) in healthy controls with nCIMT, followed by healthy controls with iCIMT, then hypertensive patients with nCIMT and the highest expression in hypertensive patients with iCIMT. Additionally, MiR-92a levels showed a significant positive correlation with 24-h mean systolic BP (r = 0.807, P < 0.001), 24-h mean diastolic BP (r = 0.649, P < 0.001), 24-h mean pulse pressure (PP) (r = 0.697, P < 0.001), 24-h daytime PP (r = 0.654, P < 0.001), 24-h nighttime PP (r = 0.573, P < 0.001), CIMT (r = 0.571, P < 0.001) and cfPWV (r = 0.601, P < 0.001). Our data present significant evidence that circulating miR-92a represents a potential noninvasive atherosclerosis marker in essential hypertensive patients.