Detection of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 2(PAI-2) in gingival crevicular fluid from healthy, gingivitis and periodontitis patients.
Detection of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 2(PAI-2) in gingival crevicular fluid from healthy, gingivitis and periodontitis patients.
复制标题
健康人、牙龈炎、牙周炎患者龈沟液中组织纤溶酶原激活物（t-PA）和纤溶酶原激活物抑制剂2（PAI-2）的检测。
DOI：
10.1034/j.1600-051x.2000.027003149.x
复制
发表时间：
2000
影响因子：
6.7
通讯作者：
P. Bartold
中科院分区：
文献类型：
--
作者：
Xiao Yin;Clive L. Bunn;P. Bartold
背景
纤溶酶原激活的调节是控制细胞外基质中蛋白分解事件的关键因素。我们以前的研究表明，在炎症牙龈组织中，结缔组织细胞外基质中的组织型纤溶酶原激活物(t-PA)显著增加，白介素1β(IL-1β)可以上调人牙龈成纤维细胞合成t-PA和纤溶酶原激活物抑制物-2(PAI-2)的水平。
方法
本研究测定了健康牙周炎、牙周炎患者和牙周炎患者牙周治疗前后牙周组织中组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制物-2(PAI-2)的水平。收集33例患者106个牙周部位的沟液。对11例牙周炎患者的24个部位进行第一次牙周治疗，治疗14天后采集治疗后样本。采用酶联免疫吸附试验(ELISA)检测t-PA和PAI-2。
结果
结果表明，在牙周炎和牙周炎部位，牙周液中t-PA和PAI-2水平明显升高。牙周治疗14天后，PAI-2水平显著下降，t-PA水平无明显变化。GCF中t-PA与PAI-2呈显著正相关(r=0.80，p<；0.01)。在健康组中，同一受试者不同部位的牙周循环液中t-PA和PAI-2变化不大。但牙周炎和牙周炎部位差异较大。这些结果提示t-PA和PAI-2与牙周疾病的严重程度有良好的相关性。
结论
提示t-PA和PAI-2在牙周组织破坏和组织改建中可能起重要作用，牙周液中t-PA和PAI-2可作为临床评价牙周疾病和疗效的指标。
BACKGROUND
The regulation of plasminogen activation is a key element in controlling proteolytic events in the extracellular matrix. Our previous studies had demonstrated that in inflamed gingival tissues, tissue-type plasminogen activator (t-PA) is significantly increased in the extracellular matrix of the connective tissue and that interleukin 1beta(IL-1beta) can up regulate the level of t-PA and plasminogen activator inhibitor-2 (PAI-2) synthesis by human gingival fibroblasts.
METHOD
In the present study, the levels of t-PA and PAI-2 in gingival crevicular fluid (GCF) were measured from healthy, gingivitis and periodontitis sites and compared before and after periodontal treatment. Crevicular fluid from106 periodontal sites in 33 patients were collected. 24 sites from 11 periodontitis patients received periodontal treatment after the first sample collection and post-treatment samples were collected 14 days after treatment. All samples were analyzed by enzyme-linked immunosorbent assay (ELISA) for t-PA and PAI-2.
RESULTS
The results showed that significantly high levels of t-PA and PAI-2 in GCF were found in the gingivitis and periodontitis sites. Periodontal treatment led to significant decreases of PAI-2, but not t-PA, after 14 days. A significant positive linear correlation was found between t-PA and PAI-2 in GCF (r=0.80, p<0.01). In the healthy group, different sites from within the same subject showed little variation of t-PA and PAI-2 in GCF. However, the gingivitis and periodontitis sites showed large variation. These results suggest a good correlation between t-PA and PAI-2 with the severity of periodontal conditions.
CONCLUSION
This study indicates that t-PA and PAI-2 may play a significant rôle in the periodontal tissue destruction and tissue remodeling and that t-PA and PAI-2 in GCF may be used as clinical markers to evaluate the periodontal diseases and assess treatment.