Identification of Free Radical Scavengers from Brazilian Green Propolis Using Off-Line HPLC-DPPH Assay and LC-MS

Identification of Free Radical Scavengers from Brazilian Green Propolis Using Off-Line HPLC-DPPH Assay and LC-MS
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使用离线 HPLC-DPPH 测定和 LC-MS 鉴定巴西绿蜂胶中的自由基清除剂

DOI:
10.1111/1750-3841.13730
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发表时间:
2017
影响因子:
3.9
通讯作者:
Shen xiaoge
Shen xiaoge
中科院分区:
农林科学3区
文献类型:
--
作者:
zhang cuiping;Shen xiaoge

文献摘要

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Brazilian green propolis is known as an appreciable natural antioxidant with abundant polyphenolic compounds. For quality control, a fingerprint‐efficacy study of Brazilian green propolis was carried out in this work. Chemical fingerprints of Brazilian green propolis from 22 different sources were determined by HPLC and investigated by similarity analysis. The fingerprint‐efficacy relationships between chemical fingerprint and DPPH radical‐scavenging activity were established. The results showed that 14 characteristic common peaks were identified, and 9 compounds were discovered with free radical‐scavenging activities. Caffeoylquinic acids and artepillin C might be the major effective components for quality control of Brazilian green propolis due to their specificity and strong antioxidant activity. This study provides new markers for the quality assessment of Brazilian green propolis and its derived products.