Sensitive HIV-1 detection in a homogeneous solution based on an electrochemical molecular beacon coupled with a nafion-graphene composite film modified screen-printed carbon electrode
Sensitive HIV-1 detection in a homogeneous solution based on an electrochemical molecular beacon coupled with a nafion-graphene composite film modified screen-printed carbon electrode
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基于电化学分子信标与 nafion-石墨烯复合膜修饰的丝网印刷碳电极相结合，在均质溶液中进行灵敏的 HIV-1 检测。
DOI：
10.1016/j.bios.2013.09.016
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发表时间：
2014-02-15
影响因子：
12.6
通讯作者：
Zheng, Lei
中科院分区：
文献类型：
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作者：
Li, Bo;Li, Zhengliang;Zheng, Lei
采用Nafion-石墨烯复合膜修饰的丝网印刷碳电极(Nafion-石墨烯/SPCE)与电化学分子信标联用，建立了一种灵敏测定均相溶液中HIV-1的电化学传感方法。电化学分子信标(cas-MB)由HIV-1 gag基因保守区的一个特殊识别序列和一对胭脂红酸分子作为标志物，可以通过其开关电化学信号行为来指示HIV-1靶标的存在。它适合于HIV-1的直接电化学检测，从而简化了检测程序，提高了信噪比(SIN)。为了进一步提高灵敏度，采用Nafion-石墨烯/SPCE监测CaS-MB的变化，具有超灵敏、廉价、一次性等显著优点。在优化条件下，峰电流与目标寡核苷酸浓度的对数在40~2.56mM范围内呈良好的线性关系，检出限为5 nM(S/N=3)。该传感方法具有良好的稳定性，回收率为88-106.8%，相对标准偏差为
A novel electrochemical sensing assay for sensitive determination of HIV-1 in a homogeneous solution has been developed using an electrochemical molecular beacon combined with a nafion-graphene composite film modified screen-printed carbon electrode (nafion-graphene/SPCE). The electrochemical molecular beacon (CAs-MB), comprising a special recognition sequence for the conserved region of the HIV-1 gag gene and a pair of carminic acid molecules as a marker, can indicate the presence of the HIV-1 target by its on/off electrochemical signal behavior. It is suitable for direct, electrochemical determination of HIV-1, thereby simplifying the detection procedure and improving the signal-to-noise (SIN) ratio. To further improve the sensitivity, the nafion-graphene/SPCE was used to monitor changes in the CAs-MB, which has notable advantages, such as being ultrasensitive, inexpensive, and disposable. Under optimized conditions, the peak currents showed a linear relationship with the logarithm of target oligonucleotide concentrations ranging from 40 nM to 2.56 mu M, with a detection limit of 5 nM (S/N=3). This sensing assay also displays a good stability, with a recovery of 88-106.8% and RSD