Effect of polyethylene glycol coatings on uptake of indocyanine green loaded nanocapsules by human spleen macrophages in vitro
Effect of polyethylene glycol coatings on uptake of indocyanine green loaded nanocapsules by human spleen macrophages in vitro
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DOI：
10.1117/1.3574761
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发表时间：
2011-05-01
影响因子：
3.5
通讯作者：
Anvari, Bahman
中科院分区：
文献类型：
--
作者：
Bahmani, Baharak;Gupta, Sharad;Anvari, Bahman
近红外(NIR)光学活性纳米粒子在医学成像和光疗等领域具有广阔的应用前景。由于纳米颗粒可以被网状内皮系统的细胞迅速从体内清除，因此用聚乙二醇(PEG)对其进行表面修饰是增加其血液循环时间的有效策略。我们构建了负载吲哚青绿(ICG)的聚合物纳米胶囊，ICG是FDA批准的一种近红外染料，并包覆了封醛的聚乙二醇膜。应用光吸收光谱和流式细胞仪技术，研究了聚乙二醇包被和聚乙二醇分子量[5000和30,000道尔顿(Da)]对含ICG纳米胶囊孵育15~360分钟的人脾巨噬细胞吞噬功能的影响。我们的结果表明，用聚乙二醇包被是一种有效的方法，可以减少ICG-NCS在巨噬细胞内的吞噬含量，孵育时间至少长达360分钟。与高相对分子质量聚乙二醇包被的ICG-NCS相比，ICG-NCS表面涂覆低相对分子质量的聚乙二醇可使ICG-NCS在巨噬细胞内的吞噬能力降低，孵育时间至少长达60分钟。聚乙二醇包覆ICG-NCS是一种在近红外成像和光疗应用中延长ICG血管循环时间的有前途的方法。(C)2011年，光学仪器工程师学会。[DOI：10.1117/1.3574761]
Near-infrared (NIR) optically active nanoparticles are promising exogenous chromophores for applications in medical imaging and phototherapy. Since nanoparticles can be rapidly eliminated from the body by cells of the reticuloendothelial system, a thriving strategy to increase their blood circulation time is through surface modification with polyethylene glycol (PEG). We constructed polymeric nanocapsules loaded with indocyanine green (ICG), an FDA-approved NIR dye, and coated with aldehyde-terminated PEG. Using optical absorbance spectroscopy and flow cytometry, we investigated the effect of PEG coating and molecular weight (MW) of PEG [5000 and 30,000 Daltons (Da)] on the phagocytic content of human spleen macrophages incubated with ICG-containing nanocapsules (ICG-NCs) between 15 to 360 min. Our results indicate that surface coating with PEG is an effective method to reduce the phagocytic content of ICG-NCs within macrophages for at least up to 360 min of incubation time. Coating the surface of ICG-NCs with the low MW PEG results in lower phagocytic content of ICG-NCs within macrophages for at least up to 60 min of incubation time as compared to ICG-NCs coated with the high MW PEG. Surface coating of ICG-NCs with PEG is a promising approach to prolong vasculature circulation time of ICG for NIR imaging and phototherapeutic applications. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3574761]