Oscillatory fluid flow affects human marrow stromal cell proliferation and differentiation
Oscillatory fluid flow affects human marrow stromal cell proliferation and differentiation
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DOI：
10.1016/j.orthres.2004.04.002
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发表时间：
2004-11-01
影响因子：
2.8
通讯作者：
Jacobs, CR
中科院分区：
文献类型：
--
作者：
Li, YJ;Batra, NN;Jacobs, CR
机械载荷是骨形成和骨丢失的重要调节因素。成骨细胞数量和功能的减少是机械废用导致骨形成减少的重要细胞机制。成骨细胞数量减少可能是骨祖细胞增殖、分化或两者减少的结果。然而，细胞水平的物理信号对骨祖细胞的影响还不清楚。在这项研究中，我们研究了负载诱导的振荡流体流（OFF），成骨细胞功能的有效调节剂，对骨髓基质细胞（MSC）的影响。经OFF处理的MSC表现出细胞内Ca2+动员增加。此外，MSC表现出增加的增殖和骨桥蛋白和骨钙素基因的mRNA水平增加。胶原蛋白I和核心结合因子1 mRNA水平没有变化。经OFF处理的MSC也表现出碱性磷酸酶活性降低。这些结果表明，骨髓间充质干细胞是机械敏感的，钙离子可能在信号通路中发挥作用。(C)2004骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
Mechanical loading is an important regulator of bone formation and bone loss. Decreased osteoblast number and function are important cellular mechanisms by which mechanical disuse leads to decreased bone formation. Decreased osteoblast number may be a result of decreased osteoprogenitor proliferation, differentiation, or both. However, the effects of cellular level physical signals on osteoprogenitors are not well understood. In this study, we examined the effects of loading induced oscillatory fluid flow (OFF), a potent regulator of osteoblastic cell function, on marrow stromal cells (MSCs). MSCs subjected to OFF exhibited increased intracellular Ca2+ mobilization. In addition, MSCs exhibited increased proliferation and increased mRNA levels for osteopontin and osteocalcin genes. Collagen I and core binding factor 1 mRNA levels did not change. MSCs subjected to OFF also exhibited decreased alkaline phosphatase activity. These results suggest that MSCs are mechanosensitive and that Ca2+ may play a role in the signaling pathway. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.