Cafeteria feeding induces interleukin-1β mRNA expression in rat liver and brain
Cafeteria feeding induces interleukin-1β mRNA expression in rat liver and brain
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DOI：
10.1152/ajpregu.1998.274.6.r1734
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发表时间：
1998-06-01
影响因子：
2.8
通讯作者：
Krueger, JM
中科院分区：
文献类型：
--
作者：
Hansen, MK;Taishi, P;Krueger, JM
食物摄入会影响肠道免疫功能，并能提供强大的肠道抗原挑战，从而激活消化系统中的宿主防御机制。在此之前，我们发现，通过隔膜下机制，给大鼠喂食自助餐会增加非快速眼动睡眠。食物摄入和睡眠调节与免疫系统共享调节分子白介素1β(IL-1β)。因此，本研究探讨了自助式饮食对大鼠肝脏和脑中IL-1β和IL-1受体辅助蛋白(IL-1RAP)mRNA表达的影响。大鼠被喂以正常的大鼠食物或由面包、巧克力和酥饼组成的可口饮食(自助餐厅饮食)。3天后，在光暗周期的中间，断头处死大鼠。与只喂食正常饮食的大鼠相比，喂食自助餐厅的大鼠肝脏和下丘脑中IL-1β的mRNA表达增加。此外，自助餐厅进食降低了肝脏和脑干中IL-1RAP mRNA的水平。这些结果表明，进食对细胞因子的产生有直接影响，与其他数据一起表明，随着进食增加而增加的睡眠可能是大脑IL-1β增加的结果。这些结果进一步表明，细胞因子与大脑的沟通在正常的生理条件下可能是重要的，如进食，以及在炎症反应中也是重要的。
Food intake affects gut-immune function and can provide a strong intestinal antigen challenge resulting in activation of host defense mechanisms in the digestive system. Previously, we showed that feeding rats a cafeteria diet increases non-rapid eye movement sleep by a subdiaphragmatic mechanism. Food intake and sleep regulation and the immune system share the regulatory molecule interleukin-1 beta (IL-1 beta). Thus this study examined the effects of a cafeteria diet on IL-1 beta mRNA and IL-1 receptor accessory protein (IL-1RAP) mRNA expression in rat liver and brain. Rats were fed normal rat chow or a palatable diet consisting of bread, chocolate, and shortbread cookies (cafeteria diet). After 3 days, midway between the light period of the light-dark cycle, rats were killed by decapitation. Feeding rats a cafeteria diet resulted in increased IL-1 beta mRNA expression in the liver and hypothalamus compared with rats fed only the normal rat chow. In addition, cafeteria feeding decreased IL-1RAP mRNA levels in the liver and brain stem. These results indicate that feeding has direct effects on cytokine production and together with other data suggest that the increased sleep that accompanies increased feeding may be the result of increased brain IL-1 beta. These results further suggest that cytokine-to-brain communication may be important in normal physiological conditions, such as feeding, as well as being important during inflammatory responses.