Identification of a gonad-expression differential gene insulin-like growth factor-1 receptor (Igf1r) in the swamp eel (Monopterus albus)
Identification of a gonad-expression differential gene insulin-like growth factor-1 receptor (Igf1r) in the swamp eel (Monopterus albus)
复制标题
沼泽鳗鱼 (Monopterus albus) 性腺表达差异基因胰岛素样生长因子 1 受体 (Igf1r) 的鉴定
DOI：
10.1007/s10695-014-9914-y
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发表时间：
2014-08-01
影响因子：
2.9
通讯作者：
He, Yan
中科院分区：
文献类型：
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作者：
Mei, Jie;Yan, Wei;He, Yan
在脊椎动物中，胚胎性腺的分化受到许多关键基因和关键类固醇合成酶的影响。胰岛素样生长因子-1受体（Insulin-like growth factor-1 receptor，Igf 1 r）是哺乳动物重要的性别分化基因，可介导Igf 1的生物学作用，是甾体激素合成关键酶的重要调节因子。然而，Igf 1 r基因在沼泽鳗鱼中仍然是未知的，沼泽鳗鱼是一种重要的经济鱼类。在本研究中，我们从黄鳝中克隆到了Igf 1 r基因，它是一个2,148 bp的开放阅读框，编码716个氨基酸。序列比对和系统进化树分析表明，黄鳝Igf 1 r基因与其它脊椎动物，尤其是鱼类的Igf 1 r基因序列比较保守。Western blotting结果显示，Igf 1 r在卵精巢和精巢中的表达量明显高于卵巢，表明Igf 1 r在性腺分化过程中起重要作用。通过免疫共沉淀分析了Igf 1 r的泛素化，发现从卵巢、卵精巢到精巢，泛素化Igf 1 r的表达量逐渐增加，这与性腺转化过程中Hsp 10的表达趋势一致。Hsp 10可能在黄鳝性腺发育过程中抑制Igf 1 r的泛素化。
In vertebrate species, the biopotential embryonic gonad differentiation is affected by many key genes and key steroidogenic enzymes. Insulin-like growth factor-1 receptor (Igf1r) has been considered as an important sex-differentiation gene in mammals and could mediate the biological action of Igf1, an important regulator of key steroidogenic enzymes. However,Igf1rgene is still unknown in the swamp eel, an economically important fish. In our study, we identifiedIgf1rgene in the swamp eel, which was a 2,148-bp open-reading frame encoding a protein of 716 amino acids. The alignment and the phylogenetic tree showed that Igf1r of the swamp eel had a conservative sequence with other vertebrates, especial fishes. Western blotting of Igf1r showed that Igf1r expressed much more in ovotestis and testis than in ovary, indicating an important role of Igf1r during gonad differentiation. We analyzed ubiquitination of Igf1r by co-immunoprecipitation and found the amount of ubiquitinated Igf1r was increased from ovary, ovotestis to testis, which was reversely to the trend of Hsp10 expression during gonadal transformation. It was possible that Hsp10 could suppress Igf1r ubiquitination during gonadal development of the swamp eel.