Merging of a chemical reaction with microbial metabolism via inverse phase transfer catalysis for efficient production of red Monascus pigments
Merging of a chemical reaction with microbial metabolism via inverse phase transfer catalysis for efficient production of red Monascus pigments
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DOI：
10.1039/c9re00179d
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发表时间：
2019-08-01
影响因子：
3.9
通讯作者：
Wang, Zhilong
中科院分区：
文献类型：
--
作者：
Gu, Bin;Xie, Haisheng;Wang, Zhilong
化学催化和微生物发酵都是生产与我们日常生活相关的小分子的重要方法。合成有机化学中使用的方法与支持活微生物所需的培养条件之间的明显不匹配仍然是在一锅法中将化学催化与微生物发酵相结合的最大挑战之一。本研究以红曲红色素的微生物发酵为模型，包括红曲红色素的生物合成和红曲红色素的非酶化学转化。以羟丙基-β-环糊精为反相转移催化剂，在水-十四烷两相体系中进行反相转移催化（IPTC），加速疏水性橙子色素的化学改性。同时，在该系统中，桔红色假单胞菌表现出良好的生物相容性。因此，微生物发酵和进一步的化学改性的橙子Monoclonal色素已成功地合并在一锅由IPTC，其中的微生物代谢通量的转移到橙子Monoclonal色素（几乎检测不到的黄色Monoclonal色素）和红色Monoclonal色素的有效生产（对应于近2克l（-1））实现。这是第一次报道利用IPTC作为一种生物相容性介质，将化学反应与微生物代谢相结合。
Both chemical catalysis and microbial fermentation are essential ways to produce small molecules related to our everyday lives. The apparent mismatch between the approaches used in synthetic organic chemistry and the culture conditions required to support living micro-organisms remains one of the largest challenges in combining chemical catalysis with microbial fermentation in a one-pot procedure. In the present work, microbial fermentation of red Monascus pigments, which involves biosynthesis of orange Monascus pigments and non-enzymatic chemical conversion of hydrophobic orange Monascus pigments into red ones, was set up as a model. Inverse phase transfer catalysis (IPTC) consisting of a water-tetradecane two-phase system with hydroxypropyl-beta-cyclodextrin as an inverse phase transfer catalyst was exploited to accelerate the chemical modification of hydrophobic orange Monascus pigments. At the same time, Monascus aurantiacus exhibited excellent biocompatibility in this system. Thus, microbial fermentation and further chemical modification of orange Monascus pigments had been merged successfully in one-pot by IPTC, where the shift of the microbial metabolic flux to orange Monascus pigments (almost undetectable yellow Monascus pigments) and efficient production of red Monascus pigments (corresponding to nearly 2 g l(-1)) were achieved. This is the first report about exploitation of IPTC as a biocompatible medium for merging of a chemical reaction with microbial metabolism.