In vitro evaluation of the direct effect of estradiol on human osteoblasts (HOB) and human mesenchymal stem cells (h-MSCs)
In vitro evaluation of the direct effect of estradiol on human osteoblasts (HOB) and human mesenchymal stem cells (h-MSCs)
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DOI：
10.1016/j.injury.2006.08.022
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发表时间：
2006-09-01
影响因子：
2.5
通讯作者：
Tsiridis, Eleftherios
中科院分区：
文献类型：
--
作者：
DiSilvio, Lucy;Jameson, Jacqueline;Tsiridis, Eleftherios
雌激素可促进成骨细胞的增殖，这取决于其分化阶段。我们的目的是验证雌二醇可以刺激原代人成骨细胞（HOB）或人间充质干细胞（h-MSCs）增殖的假设。为了验证这一假设，我们研究了两种合成雌二醇制剂：（a）市售的一种（临床使用），其效果使用MTT测定、台盼蓝细胞计数和总蛋白测定进行评价;（B）一种新的合成制剂（未在临床使用），使用Alcohol Blue测定和扫描电子显微镜（SEM）。商业雌二醇在1 nM和8 nM之间以浓度依赖性方式增加HOB增殖，在8 nM浓度下达到显著性（p < 0.001）。InM和2nM的小剂量增加了h-MSC增殖（p < 0.001），但更高剂量没有影响。所有新的合成雌二醇制剂均降低HOB增殖（p < 0.001），而对h-MSC没有观察到直接作用。商业雌二醇似乎诱导HOB的增殖，尽管其对h-MSC的作用似乎是高度剂量依赖性的，需要进一步研究。(C)2006爱思唯尔有限公司保留所有权利。
Estrogen may increase the proliferation of osteoblasts depending upon their differentiation stage. Our objective was to test the hypothesis that estradiol could stimulate the proliferation of primary human osteoblast (HOB) cells or human mesenchymal stem cells (h-MSCs). To test this hypothesis, we investigated two synthetic estradiol preparations: (a) a commercially available one (in clinical use) whose effect was evaluated using MTT assay, trypan blue cell counts and total protein assays; (b) a novel synthetic preparation (not in clinical use) using Alamar Blue assays and scanning electron microscopy (SEM). Commercial estradiol increased HOB proliferation in a concentration-dependant manner between 1 nM and 8 nM reaching significance at a concentration of 8 nM (p < 0.001). Small doses of 1 nM and 2 nM increased h-MSC proliferation (p < 0.001) but higher doses had no effect. All novel synthetic estradiol preparations decreased HOB proliferation (p < 0.001) whereas no direct effect was observed on h-MSCs. Commercial estradiol appears to induce proliferation of HOBs, although its effects on h-MSCs appears to be highly dose-dependent and requires further investigation. (C) 2006 Elsevier Ltd. All rights reserved.