Identification of ligands for olfactory receptors by functional expression of a receptor library
Identification of ligands for olfactory receptors by functional expression of a receptor library
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DOI：
10.1016/s0092-8674(00)81716-x
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发表时间：
1998-12-23
期刊：
影响因子：
64.5
通讯作者：
Reed, RR
中科院分区：
文献类型：
--
作者：
Krautwurst, D;Yau, KW;Reed, RR
嗅觉受体对气味的识别是气味辨别的第一阶段。在这里，我们报告了一个表达文库的生成，该文库包含跨膜II-VII区中的小鼠嗅觉受体序列的大型和多样化的库。从该文库中，在转染到HEK-293细胞中后，针对26种气味剂测试了80种嵌合受体。三个受体被确定为响应小分子浓度的香芹烯，（-）香茅醛，柠檬烯，分别。我们还发现，小鼠17受体，不像大鼠17受体，更喜欢庚醛，而不是辛醛，作为一个单一的缬氨酸到异亮氨酸取代的结果。这一发现代表了对气味识别的分子理解的开始。识别，在大规模上，同源受体的气味相互作用应该提供洞察嗅觉编码机制。
The recognition of odorants by olfactory receptors represents the first stage in odor discrimination. Here, we report the generation of an expression library containing a large and diverse repertoire of mouse olfactory receptor sequences in the transmembrane Il-VII region. From this library, 80 chimeric receptors were tested against 26 odorants after transfection into HEK-293 cells. Three receptors were identified to respond to micromolecular concentrations of carvene, (-) citronellal, and limonene, respectively. We also found that the mouse 17 receptor, unlike the rat 17 receptor, prefers heptanal instead of octanal, as a result of a single valine-to-isoleucine substitution. This finding represents the beginning of a molecular understanding of odorant recognition. The identification, on a large scale, of cognate receptor-odorant interactions should provide insight into olfactory coding mechanisms.