Candida duobushaemulonii: an emerging rare pathogenic yeast isolated from recurrent vulvovaginal candidiasis in Brazil.
Candida duobushaemulonii: an emerging rare pathogenic yeast isolated from recurrent vulvovaginal candidiasis in Brazil.
复制标题
DOI：
10.1590/0074-02760160166
复制
发表时间：
2016-06
影响因子：
2.8
通讯作者：
Gompertz OF
中科院分区：
文献类型：
--
作者：
Boatto HF;Cavalcanti SD;Del Negro GM;Girão MJ;Francisco EC;Ishida K;Gompertz OF
本研究的目的是鉴定从确诊为复发性外阴阴道念珠菌病(RVVC)的女性及其伴侣中分离出的念珠菌种类，并评估分离株对氟康唑(Flconazine，FLZ)的敏感性。在六年的时间里，在172例被诊断为外阴阴道念珠菌病的患者中，13名表现为RVVC的女性及其伴侣被选为本次调查的对象。用Chromagar Candida培养基获得菌株，用表型和分子方法进行菌种鉴定，用E-test法测定其对福尔马林的敏感性。从26株念珠菌中鉴定出14株白色念珠菌，6株多布沙穆隆假丝酵母菌，4株光滑假丝酵母菌和2株热带假丝酵母菌。隔离物种的一致性在100%的夫妇中出现。2株光滑假丝酵母菌耐药，所有白色念珠菌和热带假丝酵母菌对FLOZ敏感。这份报告强调了在RVVC发作中通过可靠的分子技术准确识别真菌制剂的重要性，此外，这种罕见的致病菌对FLZ的抗真菌敏感性较低。
The aim of this study was to identify Candida species isolated from women diagnosed with recurrent vulvovaginal candidiasis (RVVC) and their partners; and to evaluate the fluconazole (FLZ) susceptibility of the isolates. In a period of six years, among 172 patients diagnosed with vulvovaginal candidiasis, 13 women that presented RVVC and their partners were selected for this investigation. The isolates were obtained using Chromagar Candida medium, the species identification was performed by phenotypic and molecular methods and FLZ susceptibility was evaluated by E-test. Among 26 strains we identified 14Candida albicans, six Candida duobushaemulonii, four Candida glabrata, and twoCandida tropicalis. Agreement of the isolated species occurred in 100% of the couples. FLZ low susceptibility was observed for all isolates of C. duobushaemulonii (minimal inhibitory concentration values from 8-> 64 µg/mL), two C. glabrataisolates were FLZ-resistant and all C. albicans and C. tropicalis isolates were FLZ-susceptible. This report emphasises the importance of accurate identification of the fungal agents by a reliable molecular technique in RVVC episodes besides the lower antifungal susceptibility profile of this rare pathogen C. duobushaemulonii to FLZ.