Decreased serum levels of thioredoxin in patients with coronary artery disease plus hyperhomocysteinemia is strongly associated with the disease severity
Decreased serum levels of thioredoxin in patients with coronary artery disease plus hyperhomocysteinemia is strongly associated with the disease severity
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冠状动脉疾病合并高同型半胱氨酸血症患者血清硫氧还蛋白水平降低与疾病严重程度密切相关
DOI：
10.1016/j.atherosclerosis.2010.06.002
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发表时间：
2010-09-01
期刊：
影响因子：
5.3
通讯作者：
Zhong, Liangwei
中科院分区：
文献类型：
--
作者：
Wu, Yunfei;Yang, Lijuan;Zhong, Liangwei
目的：冠心病患者血浆同型半胱氨酸（Hcy）和硫氧还蛋白（Trx）水平升高。但与冠心病的相关性尚不清楚。硫氧还蛋白/硫氧还蛋白还原酶（TrxR）的功能障碍可引起CAD常见的氧化应激。我们试图确定同型半胱氨酸，Trx/TrxR和CAD.Methods之间的关联：血清样本收集了150例CAD患者接受他汀类药物治疗和122个非CAD对照。动脉粥样硬化的危险因素包括同型半胱氨酸、血脂和血糖水平。分别采用超胰岛素法和Western blot法测定Trx/TrxR活性和蛋白水平。采用单因素方差分析、Tukey事后检验和斯皮尔曼等级相关系数进行统计学分析。结果：与非冠心病组相比，冠心病组TrxR活性和同型半胱氨酸水平显著升高（P < 0.05和P < 0.01），而Trx活性无显著性差异。以15 μ M以下同型半胱氨酸为参照进一步划分冠心病组后，高同型半胱氨酸组Trx活性显著降低，与同型半胱氨酸水平呈负相关（r =-0.199，P < 0.05），与TrxR活性呈弱正相关。无论是脂质还是葡萄糖显着影响Trx/TrxR活性。低Trx+高同型半胱氨酸与冠心病严重程度的相关性较强（r =-0.458，P < 0.001），而与单纯高同型半胱氨酸的相关性较弱（r = 0.125，P = 0.225）。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Objective: Elevation of homocysteine and thioredoxin (Trx) levels was found in some patients with coronary artery diseases (CAD). However, their correlations with CAD were not clear. Dysfunction of thioredoxin/thioredoxin reductase (TrxR) may cause oxidative stress that is common to CAD. We seek to determine the association among homocysteine, Trx/TrxR and CAD.Methods: Serum samples were collected from 150 CAD patients under statin treatment and 122 non-CAD controls. Risk factors for atherosclerosis including homocysteine, lipids and glucose levels were analyzed. Trx/TrxR activities and protein levels were determined using super-insulin assay and Western blot, respectively. One-way ANOVA, Tukey's post hoc test and Spearman's rank correlation coefficient were used for statistical analysis. CAD severity was evaluated by angiographic Gensini score.Results: Compared with non-CAD group, CAD group had significantly increased TrxR activity (P < 0.05) and homocysteine levels (P < 0.01), but not Trxactivity. After further dividing CAD group using homocysteine below 15 mu M asreference, Trx activity decreased significantly in CAD group with high homocysteine, and was inversely associated with homocysteine levels (r = -0.199, P < 0.05) that was, however, weakly positively associated with TrxR activity. Neither lipids nor glucose significantly affected Trx/TrxR activity. Association of CAD severity with low Trx plus high homocysteine was strong (r = -0.458, P < 0.001), but with high homocysteine alone was rather weak (r = 0.125, P = 0.225).Conclusion: In CAD patients, high homocysteine levels may cause low Trx activity, which is closely correlated to the extent and severity of CAD. (C) 2010 Elsevier Ireland Ltd. All rights reserved.