Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers
Osteogenesis induction of periodontal ligament cells onto bone morphogenic protein-2 immobilized PCL fibers
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DOI：
10.1016/j.carbpol.2013.08.053
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发表时间：
2014-01-02
影响因子：
11.2
通讯作者：
Choi, Byung-Joon
中科院分区：
文献类型：
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作者：
Kim, Sung Eun;Yun, Young-Pil;Choi, Byung-Joon
本研究的目的是建立骨形态发生蛋白-2 （BMP-2）固定PCL纤维诱导牙周韧带细胞（pdlc）成骨分化。用肝素-多巴胺（Hep-DOPA） （Hep-PCL）修饰PCL纤维表面，再用BMP-2 （BMP-2/Hep-PCL）固定。利用x射线光电子能谱（XPS）和接触角对PCL纤维和表面改性的PCL纤维（Hep-PCL和BMP-2/Hep-PCL）进行了表征。碱性磷酸酶（ALP）活性、钙沉积和基因表达证实了pdlc的成骨分化。XPS和接触角结果表明，Hep-DOPA和BMP-2成功地固定在PCL表面，BMP-2/Hep-PCL纤维比单独的PCL纤维具有更好的表面亲水性。BMP-2/Hep-PCL纤维的ALP活性、钙沉积和基因表达均明显诱导了相对于PCL纤维的成骨分化。因此，我们认为BMP-2/Hep-PCL纤维具有有效诱导pdlc成骨分化的潜力。(C) 2013年Elsevier Ltd.出版
The purpose of this study was to develop bone morphogenic protein-2 (BMP-2) immobilized PCL fibers to induce osteogenic differentiation of periodontal ligament cells (PDLCs). The PCL fiber surface was modified with heparin-dopamine (Hep-DOPA) (Hep-PCL) and further immobilized with BMP-2 (BMP-2/Hep-PCL). PCL fibers and surface-modified PCL fibers (Hep-PCL and BMP-2/Hep-PCL) were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle. Osteogenic differentiation of PDLCs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and gene expression. The results of XPS and contact angle revealed that Hep-DOPA and BMP-2 were successfully immobilized onto the PCL surface and that the BMP-2/Hep-PCL fibers have more hydrophilic surface properties than PCL fibers alone. ALP activity, calcium deposition, and gene expression on BMP-2/Hep-PCL fibers showed significantly induced osteogenic differentiation relative to PCL fibers. Therefore, we suggest that BMP-2/Hep-PCL fibers have the potential to effectively induce osteogenic differentiation of PDLCs. (C) 2013 Published by Elsevier Ltd.