A bio-recognition device developed onto nano-crystals of carbonate apatite for cell-targeted gene delivery
A bio-recognition device developed onto nano-crystals of carbonate apatite for cell-targeted gene delivery
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DOI：
10.1002/bit.20398
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发表时间：
2005-05-20
影响因子：
3.8
通讯作者：
Akaike, T
中科院分区：
文献类型：
--
作者：
Chowdlhury, EH;Akaike, T
DNA递送到哺乳动物细胞是分析基因结构、调控和功能的重要工具。该方法为进一步发展基因治疗技术和DNA疫苗接种策略以治疗和控制疾病带来了巨大的希望。在这里，我们报告的建立细胞特异性基因传递和表达系统的物理吸附的细胞识别分子的纳米晶体表面的碳酸磷灰石。作为一个模型，DNA/纳米颗粒成功地与脱唾液酸胎球蛋白包被，以促进肝细胞衍生的细胞系通过脱唾液酸糖蛋白受体（ASGPr）和白蛋白的摄取，以防止与细胞表面的颗粒的非特异性相互作用。所得到的具有双重表面性质的复合颗粒可以在显着程度上加速DNA摄取和增强表达。以同样的方式涂有转铁蛋白的纳米颗粒显着增强转基因在相应的受体携带细胞中的表达，因此我们新开发的策略代表了一种普遍现象，用于将生物识别大分子锚定在磷灰石晶体表面用于靶向基因递送，在基础研究实验室中具有直接应用，并为基因治疗带来了巨大的希望。(c)2005 Wiley Periodicals，Inc.
The DNA delivery to mammalian cells is an essential tool for analyzing gene structure, regulation, and function. The approach holds great promise for the further development of gene therapy techniques and DNA vaccination strategies to treat and control diseases. Here, we report on the establishment of a cell-specific gene delivery and expression system by physical adsorption of a cell-recognition molecule on the nano-crystal surface of carbonate apatite. As a model, DNA/nano-particles were successfully coated with asialofetuin to facilitate uptake by hepatocyte-derived cell lines through the asialoglycoprotein receptor (ASGPr) and albumin to prevent nonspecific interactions of the particles with cell-surface. The resulting composite particles with dual surface properties could accelerate DNA uptake and enhance expression to a notable extent. Nano-particles coated with transferrin in the same manner dramatically enhanced transgene expression in the corresponding receptor-bearing cells and thus our newly developed strategy represents a universal phenomenon for anchoring a bio-recognition macromolecule on the apatite crystal surface for targeted gene delivery, having immediate applications in basic research laboratories and great promise for gene therapy. (c) 2005 Wiley Periodicals, Inc.