High temperature and salt stress response in French bean ( Phaseolus vulgaris )
High temperature and salt stress response in French bean ( Phaseolus vulgaris )
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发表时间：
2008
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通讯作者：
R. Nageshbabu;Devaraj Varadahally Rangaiah
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作者：
R. Nageshbabu;Devaraj Varadahally Rangaiah
非生物胁迫，如高温，和盐胁迫是影响作物产量的主要因素。高温的影响(46-48° C）和盐胁迫（0.4M）对菜豆（Phaseolus vulgaris）（一种主要蔬菜作物）的抗氧化剂和抗氧化酶的影响。这两种压力在植物中引起了类似的反应。氧化应激指标如H2 O2、TBARS、谷胱甘肽、抗坏血酸和脯氨酸显著升高。同样，抗氧化酶愈创木酚特异性过氧化物酶（POX）也显著升高.其他酶，β-淀粉酶和酸性磷酸酶（AP）活性略有增强。然而，胁迫对谷胱甘肽还原酶（GR）和过氧化氢酶（CAT）的影响相反，这是急剧下降的温度。盐胁迫下含量升高。未观察到任何变化。AP、POX和CAT同工酶。GR和β-淀粉酶同工酶谱在不同温度间存在差异。盐胁迫。SDS-PAGE显示在温度下完全不同的蛋白质组。和盐胁迫的幼苗。生长速率和鲜重的影响程度相同，相对于它们各自。对照DNA损伤在温度下更明显。比盐胁迫下的菜豆的反应机制似乎涉及到一些球员，这是共同的两种压力，很少有具体的个人压力。
Abiotic stresses, such as high temp., and salt stress are major factors which reduce crop productivity. Effects of high temp. (46-48° C) and salt stress (0.4 M) on French bean (Phaseolus vulgaris), a major vegetable crop, were evaluated in terms of antioxidants and antioxidant enzymes in S-9 cultivar. Both stresses caused similar responses in the plant. Oxidative stress indicators such as H2O2, TBARS, glutathione, ascorbic acid, and proline were significantly elevated. Similarly, antioxidant enzyme, guaiacol-specific peroxidase (POX) was significantly elevated. Other enzymes, β-amylase and acid phosphatase (AP) activities were marginally enhanced. However, stresses had contrasting effects on glutathione reductase (GR) and catalase (CAT), which were drastically reduced in temp. stress, and elevated in salt stress. No variations were obsd. in AP, POX, and CAT isoenzymes. Patterns of GR and β-amylase isoenzymes differed between temp. and salt stress. SDS-PAGE indicated entirely different sets of proteins in temp. and salt stressed seedlings. Growth rate and fresh mass were affected to same extent, relative to their resp. controls. DNA damage was more pronounced under temp. stress than under salt stress. Response mechanism of French bean appears to involve some players which are common to both the stresses, and few specific to individual stress.