Immunity to Nematospiroides dubius: parasite stages responsible for and subject to resistance in high responder (LAF1/J) mice.
Immunity to Nematospiroides dubius: parasite stages responsible for and subject to resistance in high responder (LAF1/J) mice.
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对可疑线虫的免疫：导致高反应性 (LAF1/J) 小鼠产生耐药性的寄生虫阶段。
DOI：
10.2307/3281091
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
R. Cypess
中科院分区：
文献类型：
--
作者：
R. H. Jacobson;B. O. Brooks;R. Cypess
在LAF1/J小鼠品系中，每次口服预先暴露于感染杜比氏新城疫线虫的幼虫，可使同种幼虫攻击减少90%以上；相反，单一的幼虫致敏感染对通过剖腹直接进入十二指肠的成虫没有影响。LAF1/J小鼠通过剖腹获得单次致敏感染的成虫，对感染幼虫或成虫的同源攻击均不表现出抵抗力。因为在接种挑战疫苗之前，所有致敏感染都被化疗清除了，所以“过度拥挤”对挑战感染的建立或发展的潜在影响被排除了。蠕虫特异性抗体测定表明，进入肠腔的成虫不会使宿主对幼虫或成虫的攻击产生二次反应。然而，用先前用同源幼虫感染致敏的小鼠的幼虫进行攻击，确实刺激了记忆抗体反应。总而言之，这些数据表明，在一个高响应性的小鼠品系(LAF1/J)中，幼虫阶段是刺激宿主抵抗再次感染所必需的，幼虫的挑战完全容易受到这种反应的影响。成熟的成虫显然不会刺激宿主的免疫反应，也不容易受到免疫反应的影响。
In the LAF1/J mouse strain, a single prior exposure to infective larvae of N. dubius given per os resulted in greater than 90% reduction of an homologous larval challenge; in contrast, a single larval sensitizing infection had no effect on adult worms introduced directly into the duodenum via laparotomy. The LAF1/J mice given a single sensitizing infection of adult worms via laparotomy did not exhibit resistance to homologous challenge of either infective larvae or adult worms. Because all sensitizing infections were removed chemotherapeutically before administration of challenge inocula, potential effects of "overcrowding" on establishment or development of challenge infections were precluded. Worm-specific antibody determinations indicated that adult worms introduced into the gut lumen did not prime the host for a secondary response to a challenge by larval or adult worms. However, a challenge with larvae of mice previously sensitized with an homologous larval infection, did stimulate an anamnestic antibody response. Collectively, the data indicated that in a highly responsive mouse strain (LAF1/J), larval stages were requisite in stimulation of host resistance to reinfection and a larval challenge was fully susceptible to the effects of that response. Mature adult worms apparently did not stimulate nor were they susceptible to the host's immune response.