REGULATORY ELEMENTS THAT CONTROL THE LINEAGE-SPECIFIC EXPRESSION OF MYOD
REGULATORY ELEMENTS THAT CONTROL THE LINEAGE-SPECIFIC EXPRESSION OF MYOD
复制标题
DOI：
10.1126/science.1315077
复制
发表时间：
1992-04-24
期刊：
影响因子：
56.9
通讯作者：
EMERSON, CP
中科院分区：
文献类型：
--
作者：
GOLDHAMER, DJ;FAERMAN, A;EMERSON, CP
通过分析调控肌生成决定基因myoD的DNA控制元件，研究了骨骼肌谱系决定的分子基础。远端增强子被鉴定为正调控人myoD基因的表达。myoD增强子和启动子在肌源性和几种非肌源性细胞系中具有活性。然而，在转基因小鼠胚胎中，myoD增强子和启动子共同指导lacZ转基因特异性表达骨骼肌谱系。这些数据表明，在发展过程中，myoD的调节机制，限制访问myoD控制元件的积极反式作用因子。
The molecular basis of skeletal muscle lineage determination was investigated by analyzing DNA control elements that regulate the myogenic determination gene myoD. A distal enhancer was identified that positively regulates expression of the human myoD gene. The myoD enhancer and promoter were active in myogenic and several nonmyogenic cell lines. In transgenic mouse embryos, however, the myoD enhancer and promoter together directed expression of a lacZ transgene specifically to the skeletal muscle lineage. These data suggest that during development myoD is regulated by mechanisms that restrict accessibility of myoD control elements to positive trans-acting factors.