Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immunogens. I: Protection against homologous stabilate challenge.
Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immunogens. I: Protection against homologous stabilate challenge.
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溶解的富含裂殖子的塞氏泰勒虫免疫原的免疫原性和保护功效。
DOI：
10.3347/kjp.1992.30.2.133
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
I. Kakoma
中科院分区：
文献类型：
--
作者：
B. Baek;I. Choi;B. S. Kim;R. Hansen;I. Kakoma
用低渗法从感染的红细胞中分离出瑟氏菌，用超声和差速离心纯化可溶性merozoite抗原。该制剂含有29、34、35和105 kD的免疫优势多肽。制备可溶性抗原（0.5 mg/ml），并用弗氏佐剂强化。将5个月大的韩国犊牛皮下接种该制剂，4周后再注射一次加强剂。加强剂量9周后，接种者和对照者用同源稳定物（5.6 × 10(6)红细胞/剂量，40%寄生虫血症）进行挑战。采用免疫印迹法（WB）和间接免疫荧光抗体法（IFA）检测小鼠红细胞压积、总红细胞计数、寄生虫血症和特异性抗体。接种后18周（攻毒后6周），接种牛的平均IFA滴度为1:10 240，而对照组为1:12 80。接种疫苗后，红细胞压积和总红细胞计数的变化可以忽略不计，而对照动物的血液学变化和相关贫血显著（p < 0.05）。在接种和激发后，抗体反应表明接种疫苗诱导了29和35 kD多肽的抗体的显著产生。后一种多肽被接种的动物更强烈地识别，因此它可能是潜在的候选疫苗。
Theileria sergenti were isolated from infected erythrocytes by hypotonic lysis, and soluble merozoite antigens were purified by sonication and differential centrifugation. The preparation contained 29, 34, 35 and 105 kD immuno-dominant poly-peptides. The soluble antigens (0.5 mg/ml) were prepared and fortified with Freund's adjuvant. Five month old naive Korean calves were subcutaneously inoculated with the preparation and a booster dose was administered 4 weeks later. Nine weeks after the booster dose, vaccinates and controls were challenged with a homologous stabilate (5.6 x 10(6) RBC/dose, 40% Parasitemia). All animals were monitored for hematocrit, total erythrocyte count, parasitemia and for the specific antibody by Western immunoblot (WB) and indirect immuno-fluorescent antibody (IFA) test. By 18 weeks after vaccination (6 weeks after the challenge), vaccinated cattle had an average IFA titer of 1:10,240 compared with 1:1,280 of the controls. The vaccinates showed negligible change in hematocrit and total RBC count whereas control animals showed significant (p less than 0.05) hematological changes and associated anemia. After vaccination and challenge, the antibody responses demonstrated that vaccination had induced significant production of antibody to the 29 and 35 kD polypeptides. The latter polypeptide was much more strongly recognized by the vaccinated animals, and thus it may be a potential candidate for the vaccine.