Porcine epidemic diarrhea virus uses cell-surface heparan sulfate as an attachment factor
Porcine epidemic diarrhea virus uses cell-surface heparan sulfate as an attachment factor
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猪流行性腹泻病毒利用细胞表面硫酸乙酰肝素作为附着因子
DOI：
10.1007/s00705-015-2408-0
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发表时间：
2015-07-01
影响因子：
2.7
通讯作者：
Mao, Xiang
中科院分区：
文献类型：
--
作者：
Huan, Chang-chao;Wang, Yue;Mao, Xiang
众所周知，许多病毒使用硫酸乙酰肝素作为初始附着因子。在本研究中，我们确定了猪流行性腹泻病毒（PEDV），一种新出现的兽医病毒，是否感染Vero细胞通过附着硫酸乙酰肝素。Western印迹分析、实时PCR和空斑形成试验表明，用肝素（硫酸乙酰肝素的类似物）预处理病毒时，PEDV感染被抑制。当细胞与肝素预孵育时，没有抑制作用。我们接下来证明了通过肝素酶I处理酶促去除硫酸乙酰肝素的高度硫酸化结构域抑制PEDV感染。我们还证实，氯酸钠，干扰硫酸乙酰肝素的生物合成，也抑制PEDV感染。此外，我们研究了具有不同类型硫酸化的两种肝素衍生物对PEDV感染的影响。数据表明去N-硫酸化肝素而不是N-乙酰基-去O-硫酸化肝素抑制PEDV感染。总之，我们的研究揭示了硫酸乙酰肝素在Vero细胞中充当PEDV的附着因子。
It is well known that many viruses use heparan sulfate as the initial attachment factor. In the present study, we determined whether porcine epidemic diarrhea virus (PEDV), an emerging veterinary virus, infects Vero cells by attaching to heparan sulfate. Western blot analysis, real-time PCR, and plaque formation assay revealed that PEDV infection was inhibited when the virus was pretreated with heparin (an analogue of heparan sulfate). There was no inhibitory effect when the cells were pre-incubated with heparin. We next demonstrated that enzymatic removal of the highly sulfated domain of heparan sulfate by heparinase I treatment inhibited PEDV infection. We also confirmed that sodium chlorate, which interferes with heparan sulfate biosynthesis, also inhibited PEDV infection. Furthermore, we examined the effect of two heparin derivatives with different types of sulfation on PEDV infection. The data suggested de-N-sulfated heparin, but not N-acetyl-de-O-sulfated heparin, inhibits PEDV infection. In summary, our studies revealed that heparan sulfate acts as the attachment factor of PEDV in Vero cells.