Early development of functional spatial maps in the zebrafish olfactory bulb
Early development of functional spatial maps in the zebrafish olfactory bulb
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DOI：
10.1523/jneurosci.0922-05.2005
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发表时间：
2005-06-15
影响因子：
5.3
通讯作者：
Friedrich, RW
中科院分区：
文献类型：
--
作者：
Li, J;Mack, JA;Friedrich, RW
在成年人的嗅球（OB），特定的化学类别的气味优先激活肾小球内的松散定义的区域，导致粗糙和破碎的“化学位置”的地图。在斑马鱼，氨基酸和胆汁酸主要刺激肾小球的外侧和内侧OB，分别。我们研究了这些空间反应地图在斑马鱼的发展。在受精后第3天（DPF），OB包含原肾小球结构，在随后的日子里变得精致和更多。在转基因斑马鱼系中，主要在二尖瓣细胞中表达Ca 2（+）指示蛋白反向包膜，OB中的气味反应在2.5-3 dpf时首次检测到。已经在这个阶段，氨基酸和胆汁酸诱发活动主要是在外侧和内侧OB，分别。使用合成指示剂的双光子Ca 2（+）成像用于在三维中以更高的分辨率重建活动模式。对氨基酸和胆汁酸的反应主要分别在外侧和内侧OB中检测到，几乎没有重叠。在2.5和6 dpf之间，气味反应单位的数量增加，但活动的整体空间组织持续存在。因此，一个粗略的空间组织的功能活动地图是建立在OB发展的早期肾小球尚未分化。这种空间组织在神经元回路的发育和生长过程中得以维持，并且可能对幼虫的气味处理、肾小球的分化以及后期发育阶段的活动图的细化具有重要功能。
In the adult olfactory bulb ( OB), particular chemical classes of odorants preferentially activate glomeruli within loosely defined regions, resulting in a coarse and fractured "chemotopic" map. In zebrafish, amino acids and bile acids predominantly stimulate glomeruli in the lateral and medial OB, respectively. We studied the development of these spatial response maps in zebrafish. At 3 d postfertilization (dpf), the OB contained protoglomerular structures that became refined and more numerous during subsequent days. In a transgenic zebrafish line expressing the Ca2(+) indicator protein inverse pericam, mainly in mitral cells, odor responses in the OB were first detected at 2.5-3 dpf. Already at this stage, amino acids and bile acids evoked activity predominantly in the lateral and medial OB, respectively. Two-photon Ca2(+) imaging using a synthetic indicator was used to reconstruct activity patterns at higher resolution in three dimensions. Responses to amino acids and bile acids were detected predominantly in the lateral and medial OB, respectively, with little overlap. Between 2.5 and 6 dpf, the number of odor-responsive units increased, but the overall spatial organization of activity persisted. Hence, a coarse spatial organization of functional activity maps is established very early during OB development when glomeruli are not yet differentiated. This spatial organization is maintained during development and growth of neuronal circuits and may have important functions for odor processing in larvae, for the differentiation of glomeruli, and for the refinement of activity maps at later developmental stages.