Radiation inactivation experiments predict that a large aggregate form of the insulin receptor is a highly active tyrosine-specific protein kinase.
Radiation inactivation experiments predict that a large aggregate form of the insulin receptor is a highly active tyrosine-specific protein kinase.
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辐射灭活实验预测，胰岛素受体的大聚集形式是一种高活性的酪氨酸特异性蛋白激酶。
DOI：
10.1021/bi00437a008
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Kathuria,S
中科院分区：
文献类型：
--
作者：
Fujita-Yamaguchi,Y;Harmon,JT;Kathuria,S
修订稿于 1989 年 2 月 13 日收到摘要：辐射灭活技术已用于高度纯化的胰岛素受体，以确定负责酪氨酸特异性蛋白激酶活性的功能分子大小。当用相同的受体制剂分析胰岛素结合和激酶活性时，发现激酶活性的功能大小大于胰岛素结合活性的功能大小。激酶活性的辐射灭活曲线是多相的。这表明至少有两种成分对总激酶活性有贡献。激酶的平均最小功能大小为 370000±60000 道尔顿
Revised Manuscript Received February 13, 1989 abstract: The technique of radiation inactivation has been used on a highly purified insulin receptor in order to determine the functional molecular size responsible for tyrosine-specific protein kinase activity. When both insulin binding and kinase activities were analyzed with the same receptor preparations, the functional size for kinase activity was found to be larger than that for insulin binding activity. The radiation inactivation curve for kinase activity was multiphasic. This indicates that at least two components contribute to total kinase activity. The average minimal functional size for the kinase was 370000±60000 daltons