IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF GALECTIN-3, AN IGE BINDING-PROTEIN, IN HUMAN MAST-CELLS AND BASOPHILS
IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF GALECTIN-3, AN IGE BINDING-PROTEIN, IN HUMAN MAST-CELLS AND BASOPHILS
复制标题
DOI：
10.1002/ar.1092420210
复制
发表时间：
1995-06-01
影响因子：
--
通讯作者：
SCHWARTZ, LB
中科院分区：
文献类型：
--
作者：
CRAIG, SS;KRISHNASWAMY, P;SCHWARTZ, LB
半乳糖凝集素-3是称为半乳糖凝集素的家族内的内源性可溶性凝集素，其结合β-半乳糖苷。从不同来源分离的蛋白质的同源物先前被指定为IgE结合蛋白（IgE-结合蛋白）、CEP 35、CPB 30、Mac-2、RL-29、RLL、L-29和HL-29。所有这些都被重新命名为galectin-3。这种凝集素广泛分布于小鼠、大鼠、狗、仓鼠和人的细胞和组织中，用光镜免疫组织化学和超微结构免疫金标记方法测定了半乳糖凝集素-3在几种器官的人肥大细胞、从人胎肝细胞体外发育的肥大细胞和人外周血嗜碱性粒细胞中的分布。在所有来源的肥大细胞和嗜碱性粒细胞中均观察到该蛋白的免疫标记。在细胞核和/或细胞质中检测到凝集素。核标记在异染色质上，而常染色质未被标记。细胞质标记集中在分泌颗粒上。与其他部位的肥大细胞和嗜碱性粒细胞相比，皮肤肥大细胞的染色强度通常更大。研究表明，在肥大细胞中，半乳糖凝集素-3可能参与促进它们与基底层的粘附。在这项研究中，半乳糖凝集素-3在人肥大细胞和嗜碱性粒细胞的分泌颗粒中的定位表明，这些细胞在被激活去角质时可能会释放这种凝集素。(C)1995年Wiley-Liss，Inc.
Galectin-3 is an endogenous soluble lectin within the family called galectins that bind beta-galactosides. Homologs of the protein isolated hom different sources were previously designated as IgE-binding protein (epsilon BP), CEP35, CPB30, Mac-2, RL-29, RLL, L-29, and HL-29. All are now renamed galectin-3. This lectin is widely distributed in cells and tissues of mice, rats, dogs, hamsters, and humans.Light microscopic immunohistochemistry and ultrastructural immunogold labeling methods were used to determine the distribution of galectin-3 in human mast cells of several organs, in mast cells developed in vitro from human fetal liver cells, and in human peripheral blood basophils. Immunolabeling for the protein was observed in mast cells from all sources and in basophils. The lectin was detected in the nucleus and/or the cytoplasm. The nuclear labeling was over heterochromatin whereas euchromatin was unlabeled. Cytoplasmic labeling was concentrated over secretory granules. The intensity of staining generally was greater in mast cells of skin when compared with that of mast cells in other locations and with that of basophils. Studies have indicated that in mast cells galectin-3 may be involved in promoting their adhesion to basal laminae. In this study the localization of galectin-3 in the secretory granules of human mast cells and basophils suggests that these cells may release this lectin when activated to degranulate. (C) 1995 Wiley-Liss, Inc.