Steroidogenic properties of phorbol ester and a Ca2+ ionophore in bovine adrenocortical cell suspensions.
Steroidogenic properties of phorbol ester and a Ca2+ ionophore in bovine adrenocortical cell suspensions.
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佛波酯和 Ca2 离子载体在牛肾上腺皮质细胞悬浮液中的类固醇生成特性。
DOI：
10.1016/0006-291x(84)90210-9
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发表时间：
1984
影响因子：
3.1
通讯作者：
E. Chambaz
中科院分区：
文献类型：
--
作者：
M. Culty;I. Vilgrain;E. Chambaz
当独立添加到牛肾上腺皮质束状细胞悬浮液中时，12 tetradecanoyl-phorbol-13 acetate（TPA）和Ca 2+离子载体A23187在1小时孵育期间以时间和剂量依赖性方式激活净皮质醇产生。当加在一起时（每种浓度为1 μM），药物似乎具有协同作用，并模拟了次优浓度的血管紧张素II或乙酰胆碱对这些细胞的类固醇生成作用，细胞环核苷酸水平没有可检测到的变化。此外，药物混合物显着增强乙酰胆碱的类固醇生成作用。这些观察结果表明，钙激活，磷脂依赖性蛋白激酶，这是目前在肾上腺皮质，可能被认为是一个可能的目标，在类固醇激素的作用机制，如血管紧张素和乙酰胆碱，作用于肾上腺皮质细胞通过环AMP的独立过程。
When added independently to bovine adrenocortical fasciculata cell suspensions, 12 tetradecanoyl-phorbol-13 acetate (TPA) and the Ca2+ionophore A23187 activated net cortisol production in a time and dose-dependent manner during one hour incubation. When added together (each at 1 μM concentration), the drugs appeared synergistic and mimicked the steroidogenic effect of suboptimal concentration of angiotensin II or acetylcholine on these cells, with no detectable variation of cellular cyclic nucleotide levels. In addition, the drug mixture markedly enhanced the steroidogenic effect of acetylcholine. These observations suggest that Ca2+-activated, phospholipid dependent protein kinase, which is present in adrenal cortex, might be considered as a possible target in the mechanism of action of steroidogenic agents such as angiotensin and acetylcholine, acting in adrenocortical cell through cyclic AMP independent processes.
腮腺腺泡细胞中促分泌素对磷脂酸合成的调节。
DOI：
10.1042/bj2040587
发表时间：
1982
期刊：
The Biochemical journal
影响因子：
--
作者：
Weiss,SJ;McKinney,JS;PutneyJr,JW
通讯作者：
PutneyJr,JW