Preparation of galacto-oligosaccharides using membrane
Preparation of galacto-oligosaccharides using membrane
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DOI：
10.1016/j.jfoodeng.2010.02.001
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发表时间：
2010-08-01
影响因子：
5.5
通讯作者：
Diblikova, L.
中科院分区：
文献类型：
--
作者：
Pocedicova, K.;Curda, L.;Diblikova, L.
半乳糖寡糖（GOS）具有多种生物学功能。它们是由乳糖通过一个或多个d -半乳糖单位的糖基转移到乳糖的d -半乳糖部分在-半乳糖苷酶催化下产生的。这种反应可以分批进行，也可以连续进行。采用超滤陶瓷膜（150kda）的膜反应器和间歇系统。以磷酸缓冲液中的乳糖（197.9 g L-1）、重组乳清（197 g L-1）和蒸发超滤渗透液（200.7 g L-1）为底物。Maxilact lx5000酶的使用浓度为6 U mL（-1）。间歇反应30min后，缓冲液中GOS浓度达到最高，为25.5 g L-1，重组乳清为31.1 g L-1，超滤渗透为32.9 g L-1。连续处理得到缓冲液GUS用量8.8 g L-1，重组乳清用量21.0 g L-1，超滤渗透GOS用量16.7 g L-1。(C) 2010 Elsevier Ltd.版权所有。
Galacto-oligosaccharides (GOS) have many biological functions. They are produced from lactose by a glycosyl transfer of one or more D-galactosyl units onto the D-galactose moiety of lactose catalysed by beta-galactosidase. This reaction can proceed in batch or in continuous system. Membrane reactor with ultrafiltration ceramic membrane (150 kDa) was used and batch system as well. Lactose in phosphate buffer (197.9 g L-1), recombined whey (197 g L-1) and evaporated ultrafiltration permeate (200.7 g L-1) were used as substrates. The used concentration of enzyme Maxilact LX 5000 was 6 U mL(-1). The highest concentration of GOS in buffer was reached after 30 min of batch reaction and it was 25.5 g L-1, for recombined whey it was 31.1 g L-1 and for ultrafiltration permeate it was 32.9 g L-1. For continuous process was obtained 8.8 g L-1 of GUS for buffer and 21.0 g L-1 for recombined whey and 16.7 g L-1 of GOS for ultrafiltration permeate. (C) 2010 Elsevier Ltd. All rights reserved.