Optoacoustic Imaging and Staging of Inflammation in a Murine Model of Arthritis
Optoacoustic Imaging and Staging of Inflammation in a Murine Model of Arthritis
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DOI：
10.1002/art.38642
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发表时间：
2014-08-01
影响因子：
13.3
通讯作者：
Meier, R.
中科院分区：
文献类型：
--
作者：
Beziere, N.;von Schacky, C.;Meier, R.
客观的。类风湿性关节炎 (RA) 是最常见的炎症性疾病之一，会导致受影响的关节疼痛和残疾。早期诊断对于针对症状的治疗的有效性至关重要，但其诊断需要仔细的临床、血清学和影像学检查，例如磁共振成像（MRI），这既昂贵又耗时。为了向生物医学界提供一种更容易评估关节炎进展的方法，本研究试图研究多光谱光声断层扫描 (MSOT) 在小鼠关节炎模型中的使用，通过 L-选择素/P-选择素靶向造影剂可视化体内炎症的程度。方法。将患有胶原诱导关节炎的小鼠作为 RA 模型进行研究。 MSOT 使用 L-选择素/P-选择素靶向造影剂、用近红外 (NIR) 荧光团标记的聚阴离子树枝状聚甘油硫酸酯 (dPGS) 进行，以增加关节炎关节的对比度。计算健康腿和关节炎腿之间的信号强度比。对比增强 MRI 的结果、临床观察、淋巴细胞：粒细胞比率和组织学结果作为比较的参考。结果。 MSOT 使用炎症靶向造影剂 dPGS-NIR，可以准确诊断小鼠关节的炎症。此外，使用该技术可以显着区分发炎关节和健康关节（P = 0.023）。通过临床观察、血液分析、对比增强 MRI 和离体组织学检查证实了 MSOT 图像上观察到的关节炎进展。结论。这项研究表明，炎症靶向造影剂和光声断层扫描成像的结合为 RA 的诊断和关节炎相关炎症的分期提供了一种有前途的手段。
Objective. Rheumatoid arthritis (RA) is one of the most frequent inflammatory diseases, causing pain and disability in the affected joints. Early diagnosis is essential for the efficiency of symptom-targeting treatments, but its diagnosis requires careful clinical, serologic, and imaging examinations, such as magnetic resonance imaging (MRI), which is both expensive and time consuming. In an effort to provide the biomedical community with a more accessible way to assess the advancement of arthritis, this study sought to investigate the use of multispectral optoacoustic tomography (MSOT) in a murine arthritis model, to visualize the extent of inflammation in vivo through an L-selectin/P-selectin- targeting contrast agent.Methods. Mice with collagen-induced arthritis were studied as a model of RA. MSOT was performed using an L-selectin/P-selectin-targeting contrast agent, polyanionic dendritic polyglycerol sulfate (dPGS) labeled with a near-infrared (NIR) fluorophore, to increase the contrast of the arthritic joint. The signal intensity ratios between healthy legs and arthritic legs were calculated. Findings on contrast-enhanced MRI, clinical observations, the lymphocyte: granulocyte ratio, and histologic findings served as referents for comparison.Results. MSOT using an inflammation-targeting contrast agent, dPGS-NIR, allowed for accurate diagnosis of inflammation in the mouse joints. In addition, use of this technique resulted in significant differentiation of the inflamed joints from the healthy joints (P = 0.023). The observed advancement of arthritis on the MSOT images was confirmed by clinical observation, blood analysis, contrast-enhanced MRI, and ex vivo histologic examinations.Conclusion. This study demonstrates that the combination of an inflammation-targeting contrast agent and optoacoustic tomographic imaging presents a promising means for the diagnosis of RA and the staging of arthritis-related inflammation.