Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells.
Maintenance of rat hepatocytes under inflammation by coculture with human orbital fat-derived stem cells.
复制标题
通过与人眼眶脂肪干细胞共培养维持炎症状态下的大鼠肝细胞
DOI：
10.2478/s11658-012-0004-9
复制
发表时间：
2012-06
影响因子：
8.3
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Chen X;Zhang S;Liu T;Liu Y;Wang Y
在体外保存肝细胞的功能无疑将有助于急性肝功能衰竭的管理。共培养系统可能能够防止肝细胞的功能下降。已经证明，肝细胞与骨髓间充质干细胞共培养时，可以在体外长期培养而不丧失功能。在这项研究中，人眼眶脂肪来源的干细胞分离和共培养与大鼠肝细胞。当用来自急性肝衰竭患者的血清治疗时，大鼠肝细胞单一培养物显示细胞活力降低和肝特异性功能丧失。但在共培养体系中，大鼠肝细胞仍能分泌白蛋白和合成尿素。IL-6在大鼠肝细胞与眼眶脂肪源性干细胞共培养中显著升高，可能是保护大鼠肝细胞免受炎症反应的关键免疫调节因子。我们的数据证实，眼眶脂肪来源的干细胞或其他脂肪组织来源的干细胞是体外支持大鼠肝细胞功能的理想候选者。
Preservation of hepatocyte functions in vitro will undoubtedly help the management of acute liver failure. The coculture system may be able to prevent functional decline of hepatocytes. It has already been shown that hepatocytes, when cocultured with bone marrow mesenchymal stem cells, could undergo long-term culture in vitro without loss of functions. In this study, human orbital fat-derived stem cells were isolated and cocultured with rat hepatocytes. When treated with serum from an acute liver failure patient, rat hepatocyte monoculture showed reduction of cell viability and loss of liverspecific functions. However, rat hepatocytes in the coculture system were still able to secret albumin and synthesize urea. IL-6 was significantly elevated in the coculture of rat hepatocyte with orbital fat-derived stem cells, and it might be the key immunoregulator which protects rat hepatocytes against inflammation. Our data confirmed that orbital fat-derived stem cells, or other adipose tissue-derived stem cells, are an ideal candidate to support rat hepatocyte functions in vitro.