Induced drought tolerance through wild and mutant bacterial strain Pseudomonas simiae in mung bean (Vigna radiata L.)
Induced drought tolerance through wild and mutant bacterial strain Pseudomonas simiae in mung bean (Vigna radiata L.)
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DOI：
10.1007/s11274-015-1974-3
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发表时间：
2016-01-01
影响因子：
4.1
通讯作者：
Choudhary, Devendra Kumar
中科院分区：
文献类型：
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作者：
Kumari, Sarita;Vaishnav, Anukool;Choudhary, Devendra Kumar
本研究以植物根际促生细菌（PGPR）猿猴假单胞菌（Pseudomonassimiae）菌株Au（MTCC-12057）的高产突变体为材料，对绿豆进行了耐旱性研究。用N-甲基-N-硝基-N-亚硝基胍对野生型菌株进行诱变，得到5株突变株，即AU-M1、AU-M2、AU-M3、AU-M4和AU-M5。在干旱条件下，突变株AU-M4的ACC脱氨酶（ACC-D）活性、吲哚乙酸（IAA）产量和无机磷（Pi）溶解能力均比野生株和其它4株突变株有所提高。AU-M4与野生型猴疫霉菌株Au ACC-D活性（79 nmol/mg/h）和IAA浓度（38.98 μ g/ml）相比，显示出更高的磷酸盐溶解指数（8.17）以及更高的ACC-D活性（98 nmol/mg/h）和IAA浓度（69.35 μ g/ml）。在这篇报告中，我们研究了野生型和突变型细菌菌株对干旱胁迫下绿豆植物的影响。结果表明，突变体AU-M4和野生型菌株Au接种的植株表现出上级的耐旱性，表现为植株生物量（鲜重）增加、水分含量增加、脯氨酸积累增加和渗透胁迫伤害降低。在胁迫条件下，与对照相比，突变体AU-M4和野生株Au接种的植物的乙烯水平分别降低了59%和45%。此外，细菌接种的植物表现出增强诱导的系统耐旱性，通过减少气孔大小和净光合作用，导致绿豆植物中的较高的含水量，这可能有助于植物在干旱条件下的存活。为了减轻干旱胁迫的影响，将需要使用PGPR来确保作物植物产生足够的食物。鉴于目前的领先优势，需要在这一领域开展协调一致的未来研究，特别是在应用潜在微生物进行实地评估方面。
The present study focused on the overproducing mutant of a plant growth promoting rhizobacterium (PGPR) Pseudomonas simiae strain AU (MTCC-12057) for significant drought tolerance in mung bean plants. Five mutants namely AU-M1, AU-M2, AU-M3, AU-M4 and AU-M5 were made after treatment of wild type strain with N-methyl-N-nitro-N-nitrosoguanidine. Mutant strain AU-M4 was recorded for enhanced ACC deaminase (ACC-D) activity, indole acetic acid (IAA) production and inorganic phosphate (Pi) solubilization compared to wild strain and other four mutant strains under drought condition. AU-M4 showed higher phosphate solubilization index (8.17) together with higher ACC-D activity (98 nmol/mg/h) and IAA concentration (69.35 mu g/ml) compared with the wild type P. simiae strain AU ACC-D activity (79 nmol/mg/h) and IAA concentration (38.98 mu g/ml) respectively. In this report, we investigated the effect of both wild and mutant type bacterial strain on mung bean plants under drought stress. Results showed that mutant AU-M4 and wild type strain AU inoculated plants exhibited superior tolerance against drought stress, as shown by their enhanced plant biomass (fresh weight), higher water content, higher proline accumulation and lower osmotic stress injury. Mutant AU-M4 and wild strain AU inoculated plants reduced the ethylene level by 59 and 45 % respectively, compared to the control under stress condition. Furthermore, bacterial inoculated plants showed enhanced induced systemic drought tolerance by reducing stomata size and net photosynthesis resulting higher water content in mung bean plants that may help in survival of plants during drought condition. To mitigate the effects of drought stress, use of PGPR will be needed to ensure sufficient production of food from crop plants. Taking current leads available, concerted future research is needed in this area, particularly on field evaluation with application of potential microorganisms.