INDUCTION OF A PROTEOGLYCAN CORE PROTEIN MESSENGER-RNA IN MOUSE T-LYMPHOCYTES
INDUCTION OF A PROTEOGLYCAN CORE PROTEIN MESSENGER-RNA IN MOUSE T-LYMPHOCYTES
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DOI：
10.1016/0161-5890(93)90146-3
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发表时间：
1993-06-01
影响因子：
3.6
通讯作者：
PAETKAU, V
中科院分区：
文献类型：
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作者：
ELLIOTT, JF;MILLER, CL;PAETKAU, V
小鼠T淋巴细胞系EL4.E1合成一种蛋白多糖核心蛋白(PGCP)mRNA，它与小鼠骨髓源性肥大细胞和小鼠肥大细胞瘤细胞系中发现的丝氨酸蛋白mRNA相同。佛波醇肉豆蔻酸醋酸酯对EL4·EL细胞的PGCP mRNA有较强的诱导作用，并可诱导细胞内多种细胞因子的mRNAs表达。然而，与细胞因子mRNAs的诱导相反，环孢素不能抑制PGCP mRNA的诱导。同种异体刺激正常小鼠脾细胞和ConA刺激产生IL-2的T杂交瘤细胞株也可诱导PGCP mRNA表达。其他一些细胞株表达相同或相似的mRNA，包括两个细胞毒性T细胞株和三个与骨髓源细胞相关的肿瘤细胞株。此前有报道称，在激活条件下，骨髓来源的细胞中几种蛋白多糖的水平会增加，但这似乎是第一次有报道称，通过T淋巴细胞的免疫刺激来诱导相应的PGCP mRNA。
The mouse T lymphocyte cell line EL4.E1 synthesizes a proteoglycan core protein (PGCP) mRNA which is identical to serglycin mRNA found in mouse bone marrow-derived mast cells and a mouse mastocytoma cell line. PGCP mRNA was strongly induced in EL4.El cells by phorbol myristate acetate, which also induces mRNAs for several cytokines in these cells. In contrast to the induction of cytokine mRNAs, however, the induction of PGCP mRNA was not inhibited by Cyclosporine. PGCP mRNA was also inducible by allogeneic stimulation of normal mouse spleen cells, and by Con A stimulation of an Interleukin 2-producing T hybridoma cell line. A number of other cell lines expressed an identical or similar, mRNA, including two cytotoxic T cell lines, and three tumor cell lines related to bone marrow-derived cells. The levels of several proteoglycans have previously been reported to increase in cells of bone marrow origin under activating conditions, but this appears to be the first report of an induction of the corresponding PGCP mRNA by immune stimulation of T lymphocytes.