Reducing the noise in behavioral assays: sex and age in adult zebrafish locomotion.
Reducing the noise in behavioral assays: sex and age in adult zebrafish locomotion.
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DOI：
10.1089/zeb.2012.0764
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发表时间：
2012-12
期刊：
影响因子：
2
通讯作者：
Catelyn Philpott;Corey J Donack;M. Cousin;C. Pierret
中科院分区：
文献类型：
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作者：
Catelyn Philpott;Corey J Donack;M. Cousin;C. Pierret
动物模型系统中使用了许多分析方法来测量特定的人类疾病相关行为。用成年斑马鱼和幼体斑马鱼作为行为模型越来越受欢迎。随着这项工作的进展，并有可能转化为新的治疗方法，我们必须尽最大努力通过减少混杂因素来提高这些分析的灵敏度。使用老鼠模型系统的科学家已经证明，性别和年龄可以影响许多行为。作为一个社区，他们已经采取行动，报告他们研究中使用的所有动物的年龄和性别。斑马鱼的工作还没有承担同样的任务。在这项研究中，我们评估了运动行为的性别和年龄差异。我们发现，年龄是影响运动的一个重要因素，在给定的年龄段内，性别也是如此。简而言之，随着斑马鱼年龄的增长，它们似乎表现出更少的基准线运动。在性别方面，较年轻(10个月)的斑马鱼在雄性中表现出更多的运动，而较大的斑马鱼(22个月)在雌性中表现出更多的运动。这些发现促使我们提出，那些使用斑马鱼进行行为研究的人在他们的实验设计中控制了年龄和性别，并在他们的方法中报告了这些描述符。
Many assays are used in animal model systems to measure specific human disease-related behaviors. The use of both adult and larval zebrafish as a behavioral model is gaining popularity. As this work progresses and potentially translates into new treatments, we must do our best to improve the sensitivity of these assays by reducing confounding factors. Scientists who use the mouse model system have demonstrated that sex and age can influence a number of behaviors. As a community, they have moved to report the age and sex of all animals used in their studies. Zebrafish work does not yet carry the same mandate. In this study, we evaluated sex and age differences in locomotion behavior. We found that age was a significant factor in locomotion, as was sex within a given age group. In short, as zebrafish age, they appear to show less base level locomotion. With regard to sex, younger (10 months) zebrafish showed more locomotion in males, while older zebrafish (22 months) showed more movement in females. These findings have led us to suggest that those using the zebrafish for behavioral studies control for age and sex within their experimental design and report these descriptors in their methods.