COMPLETE STRUCTURE OF A-DUCK-UKRAINE-63 INFLUENZA HEMAGGLUTININ GENE - ANIMAL VIRUS AS PROGENITOR OF HUMAN-H3 HONG-KONG 1968 INFLUENZA HEMAGGLUTININ
COMPLETE STRUCTURE OF A-DUCK-UKRAINE-63 INFLUENZA HEMAGGLUTININ GENE - ANIMAL VIRUS AS PROGENITOR OF HUMAN-H3 HONG-KONG 1968 INFLUENZA HEMAGGLUTININ
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DOI：
10.1016/0092-8674(81)90049-0
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发表时间：
1981-01-01
期刊：
影响因子：
64.5
通讯作者：
FIERS, W
中科院分区：
文献类型：
--
作者：
FANG, RX;JOU, WM;FIERS, W
研究了动物病毒储库含有自1968年以来人类甲型流感病毒中存在的H3血凝素类型的直接祖先基因的可能性。为此目的，鸭/乌克兰/1/63血凝素基因的克隆和测序。通过将其完整一级结构与几种人H3血凝素以及H2和H7血凝素的完整一级结构进行比较，可以得出结论，鸭/乌克兰/63血凝素序列完全证实了其先前通过免疫学和其他方法进行的鉴定属于H3亚型。鸭/Ukraine/63氨基酸序列与人Aichi/68血凝素在结构上和抗原性上比维多利亚/75更密切相关，后者在人类中经历了额外的7年漂移期，Aichi/68血凝素形成了人类H3 N2大流行的开始。这可能是最好的解释鸭/乌克兰/63和人类爱知/68的共同祖先血凝素基因。根据沉默的累积碱基变化，估计携带这种假定的共同祖细胞血凝素基因的菌株在1949-1953年期间在动物宿主中流行。与后来的维多利亚/75相比，鸭/乌克兰/63和人Aichi/68血凝素之间的这种相对较新的分歧以及更近的亲缘关系强烈表明，自1968年以来在人群中流行的H3 N2亚型甲型流感病毒的血凝素基因来源于动物宿主中的一种毒株。毫无疑问，这是由于先前存在的人H2 N2病毒与这种动物毒株之间的重配而发生的。已知存在于动物体内的多种流感病毒显然可以作为人类甲型流感病毒的基因库。
The possibility that an animal viral reservoir contained a direct ancestor gene for the H3 hemagglutinin type present in influenza A viruses in humans since 1968 was investigated. For this purpose, the duck/Ukraine/1/63 hemagglutinin gene was cloned and sequenced. From the comparison of its complete primary structure with that of several human H3 hemagglutinins and those of an H2 and an H7 hemagglutinin, it was concluded that the duck/Ukraine/63 hemagglutinin sequence fully corroborates its previous identification by immunological and other methods as belonging to the H3 subtype. The duck/Ukraine/63 amino acid sequence is more closely related structurally and presumably antigenically to the human Aichi/68 hemagglutinin, which formed the beginning of the H3N2 pandemic in humans, than to that of Victoria/75, which has undergone an additional 7 yr drift period in humans. This could best be explained by a common ancestor hemagglutinin gene for duck/Ukraine/63 and human Aichi/68. On the basis of silent, accumulated base changes, it was estimated that the strain carrying this postulated common progenitor hemagglutinin gene was circulating in the period 1949-1953 in the animal reservoir. This relatively recent divergence, and the closer kinship between the duck/Ukraine/63 and the human Aichi/68 hemagglutinin, as compared with the later Victoria/75, strongly suggests that the influenza A virus of the H3N2 subtype circulating in the human population since 1968 has derived its hemagglutinin gene from a strain in the animal reservoir. Undoubtedly, this occurred by reassortment between previously present human H2N2 virus and this animal strain. The wide variety of influenza viruses known to be present in animals can evidently serve as a gene reservoir for human influenza A viruses.