Intercellular protein movement in syncytial Drosophila follicle cells
Intercellular protein movement in syncytial Drosophila follicle cells
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DOI：
10.1242/jcs.090456
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Cooley, Lynn
中科院分区：
文献类型：
--
作者：
Airoldi, Stephanie J.;McLean, Peter F.;Cooley, Lynn
连接果蝇种系、卵泡和成虫盘细胞的环管提供细胞间细胞质的直接接触。迄今为止，人们对卵泡和成虫盘细胞中体细胞环管的形成、结构或功能知之甚少。在这里，我们通过共聚焦和电子显微镜证明帕瓦罗蒂驱动蛋白样蛋白和 Visgun 是体细胞环管的稳定成分。使用活细胞共聚焦显微镜，我们发现体细胞环管是由有丝分裂沟的稳定形成的。与生殖系细胞相反，合胞体卵泡细胞不同步分裂，没有最大程度地分支，并且在卵室发育过程中它们的环管尺寸不会增加。我们首次证明体细胞环管允许卵泡细胞之间交换细胞质蛋白。这些结果提供了对体细胞环管的组成和功能的深入了解，这意味着生殖系外细胞的合胞体组织具有更广泛的功能意义。
Ring canals connecting Drosophila germline, follicle and imaginal disc cells provide direct contact of cytoplasm between cells. To date, little is known about the formation, structure, or function of the somatic ring canals present in follicle and imaginal disc cells. Here, we show by confocal and electron microscopy that Pavarotti kinesin-like protein and Visgun are stable components of somatic ring canals. Using live-cell confocal microscopy, we show that somatic ring canals form from the stabilization of mitotic cleavage furrows. In contrast to germline cells, syncytial follicle cells do not divide synchronously, are not maximally branched and their ring canals do not increase in size during egg chamber development. We show for the first time that somatic ring canals permit exchange of cytoplasmic proteins between follicle cells. These results provide insight into the composition and function of ring canals in somatic cells, implying a broader functional significance for syncytial organization of cells outside the germline.