Fluorochrome-functionalized nanoparticles for imaging DNA in biological systems.
Fluorochrome-functionalized nanoparticles for imaging DNA in biological systems.
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DOI：
10.1021/nn305962n
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发表时间：
2013-03-26
期刊：
影响因子：
17.1
通讯作者：
Josephson, Lee
中科院分区：
文献类型：
--
作者：
Cho, Hoonsung;Alcantara, David;Yuan, Hushan;Sheth, Rahul A.;Chen, Howard H.;Huang, Peng;Andersson, Sean B.;Sosnovik, David E.;Mahmood, Umar;Josephson, Lee
将DNA结合的荧光染料结合到纳米颗粒(NPs)上提供了一种通过荧光介导的相互作用获得与DNA结合的NPs的方法。为了在生物体系中获得与DNA结合的纳米粒子(NP)，我们将DNA结合的荧光色素To-pro1(TO)连接到Feraheme(FH)NP的表面，得到了标记为TO-FH的荧光色素功能化的NP。当在体外与DNA反应时，TO-FH形成微聚体，其特征是荧光、光散射和T2变化。用原子力显微镜研究了DNA/to-FH微聚体的形成，微聚体的平均尺寸为200 nm，由DNA和多个直径仅为25-35 nm的to-FH纳米颗粒组成。在培养中，TO-FH不能与正常细胞结合，但用化疗药物或洗涤剂处理后，可以产生与-FH和重要荧光蛋白结合的坏死细胞。表面荧光和MRI显示，经5-FU和奥沙利铂处理的HT-29异种移植瘤对TO-FH有明显的摄取。将多个DNA结合的荧光染料附着在磁性纳米颗粒上提供了一种产生DNA结合NPs的方法，该NPs可用于通过体外微团聚体的形成来检测DNA，用于成像培养中的坏死细胞的DNA，以及用于成像经化疗药物治疗的肿瘤的DNA。荧光功能化纳米粒子是一种多模式(磁性和荧光)、高度多价(n≈10氟铬/NP)的纳米材料，可用于对生物系统的DNA进行成像。
Attaching DNA binding fluorochromes to nanoparticles (NPs) provides a way of obtaining NPs that bind to DNA through fluorochrome mediated interactions. To obtain a nanoparticle (NP) that bound to the DNA in biological systems, we attached the DNA binding fluorochrome, TO-PRO 1 (TO), to the surface of the Feraheme (FH) NP, to obtain a fluorochrome-functionalized NP denoted TO-FH. When reacted with DNA in vitro, TO-FH formed microaggregates that were characterized by fluorescence, light scattering, and T2 changes. The formation of DNA/TO-FH microaggregates was also characterized by AFM, with microaggregates exhibiting a median size of 200 nm, and consisting of DNA and multiple TO-FH NPs whose individual diameters were only 25–35 nm. TO-FH failed to bind normal cells in culture, but treatment with chemotherapeutic agents or detergents yielded necrotic cells that bound TO-FH and vital fluorochromes similarly. The uptake of TO-FH by HT-29 xenografts (treated with 5-FU and oxaliplatin) was evident by surface fluorescence and MRI. Attaching multiple DNA binding fluorochromes to magnetic nanoparticles provides a way of generating DNA binding NPs that can be used to detect DNA detection by microaggregate formation in vitro, for imaging the DNA of necrotic cells in culture, and for imaging the DNA of a tumor treated with a chemotherapeutic agent. Fluorochrome functionalized NPs are a multimodal (magnetic and fluorescent), highly multivalent (n ≈ 10 fluorochromes/NP) nanomaterials useful for imaging the DNA of biological systems.
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DOI：
10.1039/b907375b
发表时间：
2009-08-07
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Garanger E;Hilderbrand SA;Blois JT;Sosnovik DE;Weissleder R;Josephson L
通讯作者：
Josephson L
影响因子：
10.3
作者：
Dreher, MR;Liu, WG;Chilkoti, A
通讯作者：
Chilkoti, A
影响因子：
7.3
作者：
Galande, Amit K.;Hilderbrand, Scott A.;Tung, Ching-Hsuan
通讯作者：
Tung, Ching-Hsuan
影响因子：
19.7
作者：
Lutz, AM;Weishaupt, D;Kaim, AH
通讯作者：
Kaim, AH
影响因子：
3.2
作者：
Perez, JM;Josephson, L;Weissleder, R
通讯作者：
Weissleder, R