Isolation of Trametes hirsuta La-7 with high laccase-productivity and its application in metabolism of 17β-estradiol.
Isolation of Trametes hirsuta La-7 with high laccase-productivity and its application in metabolism of 17β-estradiol.
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DOI：
10.1016/j.envpol.2020.114381
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发表时间：
2020-03
影响因子：
8.9
通讯作者：
Kai Sun;Xing Cheng;Jialin Yu;Luojian Chen;Jiajun Wei;Wenjun Chen;Jun Wang;Shunyao Li;Qingzhu Liu;Y. Si
中科院分区：
文献类型：
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作者：
Kai Sun;Xing Cheng;Jialin Yu;Luojian Chen;Jiajun Wei;Wenjun Chen;Jun Wang;Shunyao Li;Qingzhu Liu;Y. Si
从污染土壤中分离到一株粗毛栓菌（Trametes hirsuta La-7），在优化的发酵条件下，其漆酶活力达到65.4 U· mL− 1;利用高分辨质谱结合13 C-同位素标记技术对E2代谢产物进行了鉴定，发现E2通过自由基偶联作用逐步聚合产生多种低聚物; CC-ThLac能够在环境基质中有效代谢E2。
HighlightsA Trametes hirsuta La-7 was isolated from contaminated soil to yield laccase.Activity of laccase reached 65.4 U· mL− 1 under the optimal fermentation conditions.E2 metabolites were identified by HRMS combined with 13 C-isotope labeling.E2 was step-polymerized to produce various oligomers via radical coupling mechanism.CC-ThLac was capable of effectively metabolizing E2 in environmental matrices.