Iron and citrate export by a major facilitator superfamily pump regulates metabolism and stress resistance in Salmonella Typhimurium
Iron and citrate export by a major facilitator superfamily pump regulates metabolism and stress resistance in Salmonella Typhimurium
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DOI：
10.1073/pnas.1218274110
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发表时间：
2013-07-16
影响因子：
11.1
通讯作者：
Fang, Ferric C.
中科院分区：
文献类型：
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作者：
Frawley, Elaine R.;Crouch, Marie-Laure V.;Fang, Ferric C.
抗生素和宿主防御的功效与细菌的代谢和氧化还原状态有关。在这项研究中，我们报告说，应力诱导的出口泵属于主要的促进剂超家族流出柠檬酸盐和铁从肠道病原体鼠伤寒沙门氏菌逮捕生长和改善抗生素，过氧化氢和一氧化氮的影响。转运蛋白，以前称为MdtD，现在被命名为IceT（柠檬酸铁外排转运蛋白）。通过铁螯合三羧酸循环中间体的铁流出提供了有氧代谢和细菌应激反应之间的直接联系，代表了对不同类别的宿主防御和抗菌剂的抗性的独特机制。
The efficacy of antibiotics and host defenses has been linked to the metabolic and redox states of bacteria. In this study we report that a stress-induced export pump belonging to the major facilitator superfamily effluxes citrate and iron from the enteric pathogen Salmonella Typhimurium to arrest growth and ameliorate the effects of antibiotics, hydrogen peroxide, and nitric oxide. The transporter, formerly known as MdtD, is now designated IceT (iron citrate efflux transporter). Iron efflux via an iron-chelating tricarboxylic acid cycle intermediate provides a direct link between aerobic metabolism and bacterial stress responses, representing a unique mechanism of resistance to host defenses and antimicrobial agents of diverse classes.