Application of electrospray ionization ion trap/time-of-flight mass spectrometry for chemically-synthesized small RNAs
Application of electrospray ionization ion trap/time-of-flight mass spectrometry for chemically-synthesized small RNAs
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DOI：
10.1016/j.jbiosc.2011.11.007
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发表时间：
2012-03-01
影响因子：
2.8
通讯作者：
Fukusaki, Eiichiro
中科院分区：
文献类型：
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作者：
Izumi, Yoshihiro;Takimura, Shin;Fukusaki, Eiichiro
在这项研究中，我们已经证明了一个准确和快速的小RNA分析方法，序列测定和详细的修改分析电喷雾离子阱/飞行时间质谱（ESI-IT/TOFMS）。为了开发这种理想的方法，我们使用各种化学合成的修饰或未修饰的microRNA（miRNA）的模型序列检查了ESI-IT/TOFMS的性能。从多电荷前体离子（MS 1）获得22个核苷酸（nt）miRNA的去卷积质量。离子显示出高质量精度（
In this study, we have demonstrated an accurate and rapid small RNA analytical method with both sequence determination and detailed modification analysis by electrospray ionization-ion trap/time-of-flight mass spectrometry (ESI-IT/TOFMS). To develop this ideal method, we have examined the performance of ESI-IT/TOFMS using various chemically-synthesized model sequences of modified or unmodified microRNAs (miRNAs). The deconvoluted mass of a 22-nucleotide (nt) miRNA was obtained from a multiply charged precursor ion (MS1). The ion exhibited high mass accuracy (