EXCRETED ADENOSINE IS A CELL-DENSITY SIGNAL FOR THE INITIATION OF FRUITING BODY FORMATION IN MYXOCOCCUS-XANTHUS
EXCRETED ADENOSINE IS A CELL-DENSITY SIGNAL FOR THE INITIATION OF FRUITING BODY FORMATION IN MYXOCOCCUS-XANTHUS
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DOI：
10.1016/0012-1606(81)90369-9
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
DWORKIN, M
中科院分区：
文献类型：
--
作者：
SHIMKETS, LJ;DWORKIN, M
M. xanthus形成多细胞子实体响应饥饿。子实体形成的起始依赖于细胞密度。细胞显然通过滴定分泌的腺苷的细胞外浓度来测量它们的细胞密度。在低细胞密度下，除非添加腺苷，否则不会发生子实体形成。Norit是一种结合嘌呤的物质，抑制子实体的形成，这种抑制作用被腺苷逆转。用[14 C]碳酸盐标记的细胞分泌与Norit结合的[14 C]腺苷。[14C]腺苷由发育中的细胞分泌，其浓度将在低细胞密度下诱导子实体形成。细胞外腺苷浓度在很宽的密度范围内随着细胞密度而增加。Hadacidin是一种AMP从头合成的抑制剂，抑制子实体的形成，并且Hadacidin的抑制作用可以用腺苷逆转。腺苷似乎也参与聚集过程，因为子实体的形状和大小对腺苷的外部浓度敏感。
M. xanthus forms multicellular fruiting bodies in response to starvation. The initiation of fruiting body formation is cell density dependent. Cells apparently measure their cell density by titering the extracellular concentration of excreted adenosine. At low cell densities, fruiting body formation does not occur unless adenosine is added. Norit, a substance that binds purines, inhibits fruiting body formation, and this inhibition is reversed by adenosine. Cells labeled with [14C]carbonate excrete [14C]adenosine which is bound by the Norit. [14C]Adenosine is excreted by developing cells at a concentration that will induce fruiting body formation at low cell density. The extracellular adenosine concentration increases with the cell density over a broad range of densities. Hadacidin, an inhibitor of de novo AMP synthesis, inhibits fruiting body formation, and inhibition by hadacidin can be reversed with adenosine. Adenosine also appears to be involved in the aggregation process because the shape and size of the fruiting bodies are sensitive to the external concentration of adenosine.