Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research
Serum, plasma, and dried blood spot high-sensitivity C-reactive protein enzyme immunoassay for population research
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DOI：
10.1016/j.jim.2010.09.014
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发表时间：
2010-10-31
影响因子：
2.2
通讯作者：
O'Connor, Kathleen A.
中科院分区：
文献类型：
--
作者：
Brindle, Eleanor;Fujita, Masako;O'Connor, Kathleen A.
C-反应蛋白（CRP）在人群健康研究中被用作发病率和死亡率风险的生物标志物，并且对于解释几种微量营养素生物标志物至关重要。因此，需要一种用于大规模非临床研究的稳健的高灵敏度CRP（hsCRP）测量方法。我们开发了一种高效、廉价的检测方法，适用于使用任何血液样本类型在生理范围内定量CRP。ELISA使用容易获得的单克隆抗体来测量血清、血浆或由静脉血或毛细血管血制成的干血斑（DBS）中的CRP。通过标准方法评价测定性能，包括与先前描述的测定进行比较。通过测量52份匹配的血清、血浆、静脉和毛细血管干血斑标本中的CRP，测试标本类型的影响。评价了CRP的长期和短期稳定性。检测限、线性、回收率、不精密度和与已建立方法的一致性（Pearson相关系数= 0.988，n = 20）的评估证明了新测定法的有效性。血清、血浆和DBS中的CRP测量值的Pearson相关性为0.974 - 0.995，n = 52，但血清中的CRP平均比DBS高1.6倍（SD 0.37）。CRP在冷冻血清中稳定长达34个月，但DBS CRP在暴露于环境温度和-20 ℃长期储存时迅速下降。该hsCRP检测试剂盒是一种专为大规模人群健康研究而设计的耐用且廉价的工具。我们的研究结果表明，DBS CRP的稳定性低于以前的报道。(C)2010年爱思唯尔。All rights reserved.
C-reactive protein (CRP) is used as a biomarker of morbidity and mortality risk in studies of population health, and is essential to interpretation of several micronutrient biomarkers. There is thus a need for a robust high-sensitivity CRP (hsCRP) measurement method for large-scale, non-clinical studies. We developed an efficient, inexpensive assay suitable for quantifying CRP across the physiological range using any blood specimen type. The ELISA uses readily available monoclonal antibodies to measure CRP in serum, plasma, or dried blood spots (DBS) made from venous or capillary blood. Assay performance was evaluated by standard methods, including comparison with a previously described assay. Effects of specimen type were tested by measuring CRP in 52 matched serum, plasma, and venous and capillary dried blood spot specimens. Long- and short-term CRP stability were evaluated. Assessments of assay limits of detection, linearity, recovery, imprecision, and concordance with an established method (Pearson correlation = 0.988, n = 20) demonstrated the validity of the new assay. CRP measurements in serum, plasma, and DBS had Pearson correlations from 0.974 to 0.995, n = 52, but CRP in serum was on average 1.6 times (SD 0.37) higher than in DBS. CRP was stable in frozen serum for up to 34 months, but DBS CRP declined quickly with exposure to ambient temperatures, and across long-term storage at -20 degrees C. This hsCRP assay is a robust and inexpensive tool designed for use in large-scale population health research. Our results indicate that DBS CRP is less stable than previously reported. (C) 2010 Elsevier By. All rights reserved.