Rapid Bead-Based Antimicrobial Susceptibility Testing by Optical Diffusometry.
Rapid Bead-Based Antimicrobial Susceptibility Testing by Optical Diffusometry.
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DOI：
10.1371/journal.pone.0148864
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Chuang HS
中科院分区：
文献类型：
--
作者：
Chung CY;Wang JC;Chuang HS
本研究结合光学扩散法和基于珠的免疫测定，开发了一种新的技术，用于定量特定微生物的生长，并实现快速AST。当活细菌附着在颗粒上时，扩散率上升，导致来自运动微生物的额外能量。然而，当UV灭菌（死亡）细菌附着在颗粒上时，扩散率下降。实验数据与根据当量体积直径预测的理论模型一致。使用该扩散测量平台，测试了铜绿假单胞菌对抗生素庆大霉素的敏感性。结果表明，庆大霉素能有效地抑制细菌的增殖。本研究证明了一种灵敏（单个颗粒上的一种细菌）和省时（2小时内）的平台，具有小样本体积（约0.5 μL）和低初始细菌计数（50 CFU/液滴~ 105 CFU/mL），可根据布朗运动定量微生物的生长。该技术可以进一步应用于其他细菌菌株，并在不久的将来增加对感染性疾病的治疗成功率。
This study combined optical diffusometry and bead-based immunoassays to develop a novel technique for quantifying the growth of specific microorganisms and achieving rapid AST. Diffusivity rises when live bacteria attach to particles, resulting in additional energy from motile microorganisms. However, when UV-sterilized (dead) bacteria attach to particles, diffusivity declines. The experimental data are consistent with the theoretical model predicted according to the equivalent volume diameter. Using this diffusometric platform, the susceptibility of Pseudomonas aeruginosa to the antibiotic gentamicin was tested. The result suggests that the proliferation of bacteria is effectively controlled by gentamicin. This study demonstrated a sensitive (one bacterium on single particles) and time-saving (within 2 h) platform with a small sample volume (~0.5 μL) and a low initial bacteria count (50 CFU per droplet ~ 105 CFU/mL) for quantifying the growth of microorganisms depending on Brownian motion. The technique can be applied further to other bacterial strains and increase the success of treatments against infectious diseases in the near future.