Oocyte all-surfaces’ imaging method using micro-scale rotational flow
Oocyte all-surfaces’ imaging method using micro-scale rotational flow
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微尺度旋转流卵母细胞全表面成像方法
DOI：
10.1049/mnl.2017.0731
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发表时间：
2018
影响因子：
1.3
通讯作者：
Tanaka Yo
中科院分区：
文献类型：
--
作者：
Yalikun Yaxiaer;Aishan Yusufu;Mosha Abulaiti;Sumiyama Kenta;Tanaka Yo
在这项工作中，作者报告了一种使用微尺度旋转流的单个卵母细胞的全表面图像捕获方法。在这里，作者使用这种方法轻轻地间接旋转单个原核受精卵（物理性质与卵母细胞相似），在0.33秒内获得其所有表面的图像。实现这种方法的必要设备仅包括一个注射泵和一个具有单个40 µm直径孔的微流体芯片。在细胞质内单精子注射和体外受精过程中评估卵母细胞或胚胎的活力和质量对于良好的胚胎发育是重要的。目前，人工形态学评价是评价卵母细胞活力和质量的最常用方法。通常，在人工形态学评价过程中，操作者需要操纵卵母细胞以获得其全表面图像。然而，即使对于有经验的操作者，操作也是困难的。使用机械、电子或磁性原理的常规方法需要复杂的系统。使用流体或光学原理的常规方法也具有关于目标尺寸和透明度的限制。本文提出并证明了一种利用旋转流来轻柔地捕获和旋转小鼠受精卵的方法，而不需要复杂的控制系统，并在0.33 s内实现了受精卵的全表面成像。该方法为评价卵母细胞的存活率和质量提供了一种新的形态学评价工具。
In this work, the authors report an all‐surfaces’ image capturing method of a single oocyte that uses micro‐scale rotational flow. Here, the authors used this method gently and indirectly rotated the single pronuclear zygotes (physical properties are similar to those of oocytes) to obtain images of all its surfaces in 0.33 s. The necessary equipment to realise this method consists of only a syringe pump and a microfluidic chip with a single 40 µm diameter orifice. Assessment of viability and quality of oocytes or embryos during the process of intra cytoplasmic sperm injection) andin vitrofertilisation is important for good embryo development. At present, manual morphological evaluation is the most common method to assess viability and quality of oocytes. Usually, during the manual morphological evaluation, the operator is required to manipulate the oocyte to achieve its full surface image. However, manipulation is difficult, even for an experienced operator. Conventional methods using the principles of mechanics, electronics, or magnetism require a complex system. Conventional methods using principles of fluids or optics also have limitations about target size and transparency. Here, a method is proposed and proved of using rotational flow to gently capture and rotate a mouse zygote without using a complex control system and achieved its full surface image in 0.33 s. The method offers a new tool for morphological evaluation of assessment of viability and quality of oocytes.