Establishment and characterization of a human Epstein-Barr virus-associated gastric carcinoma in SCID mice
Establishment and characterization of a human Epstein-Barr virus-associated gastric carcinoma in SCID mice
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DOI：
10.1128/jvi.72.10.8321-8326.1998
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发表时间：
1998-10-01
影响因子：
5.4
通讯作者：
Fukayama, M
中科院分区：
文献类型：
--
作者：
Iwasaki, Y;Chong, JM;Fukayama, M
人EB病毒相关性胃癌(EBVaGC)在严重联合免疫缺陷(SCID)小鼠体内传代12代，命名为KT。粘蛋白和细胞角蛋白的表达以及肿瘤DNA中的Alu序列证实了KT肿瘤来源于人上皮组织。通过对EBV DNA的末端重复序列分析，证实了克隆EBV在原发肿瘤和KT肿瘤中的一致性。EBV潜伏基因在两种肿瘤中的表达模式相同。原位杂交显示EBER1在肿瘤细胞核中也有类似的表达。逆转录-聚合酶链式反应分析也证实了Q启动子驱动的EBNA1的表达，但没有发现BZLF1、EBNA2或LMP1的表达。因此，可移植的人EBVaGC KT保持了原始EBV的潜伏期基因表达，可以作为这种独特类型的胃癌的模型。
A transplantable human Epstein-Barr virus-associated gastric carcinoma (EBVaGC), designated KT, was propagated in severe combined immunodeficiency (SCID) mice for 12 passages. Mucin and cytokeratin expression and the Alu sequence in tumor DNA confirmed that the KT tumor was derived from human epithelial tissue. The identity of clonal EBV in the original and KT tumors was demonstrated by terminal repeat analysis of EBV DNA. The pattern of latency gene expression of EBV was the same in both tumors. EBER1 was presented similarly in tumor cell nuclei by in situ hybridization. Reverse transcription-PCR analysis also demonstrated Q-promoter-driven EBNA1 expression but not BZLF1, EBNA2, or LMP1 expression. Thus, the transplantable human EBVaGC KT retains the original EBV with the same latency gene expression and can serve as a model for this unique type of gastric carcinoma.