TRANSPORT OF CYSTINE AND CYSTEINE AND CELL-GROWTH IN CULTURED HUMAN-DIPLOID FIBROBLASTS - EFFECT OF GLUTAMATE AND HOMOCYSTEATE
TRANSPORT OF CYSTINE AND CYSTEINE AND CELL-GROWTH IN CULTURED HUMAN-DIPLOID FIBROBLASTS - EFFECT OF GLUTAMATE AND HOMOCYSTEATE
复制标题
DOI：
10.1002/jcp.1041120216
复制
发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
ISHII, T
中科院分区：
文献类型：
--
作者：
BANNAI, S;ISHII, T
人二倍体成纤维细胞（1MR-90细胞）在培养基中吸收胱氨酸，胱氨酸在细胞中立即还原为半胱氨酸。以这种方式形成的半胱氨酸迅速从细胞中释放到培养基中并在那里积累。运输半胱氨酸的系统与Christensen等人（1967）描述的ASC系统令人信服地相似。由于培养基中的半胱氨酸对自氧化很敏感，很容易变回胱氨酸，因此对胱氨酸的摄取似乎对细胞至关重要。半胱氨酸摄取抑制剂，如谷氨酸和同型半胱氨酸，能有效降低半胱氨酸在细胞内和细胞外的水平。这些抑制剂根据培养基中的胱氨酸浓度改变细胞生长。当胱氨酸浓度达到生理水平时，它们抑制生长。过高浓度的胱氨酸本身具有生长抑制作用，但这种抑制作用可被谷氨酸或同型半胱氨酸拮抗。
Human diploid fibroblasts (1MR-90 cell) take up cystine in the culture medium and the cystine is immediately reduced to cysteine in the cells. Cysteine formed in this way is rapidly released from the cells into the medium and accumulates there. The system transporting cysteine is convincingly similar to the ASC system described by Christensen et al. (1967). Since cysteine in the medium is sensitive to autoxidation and readily changes back to cystine, the uptake of cystine seems crucial to the cells. Inhibitors of cystine uptake, such as glutamate and homocysteate, potently reduce the intracellular and extracellular levels of cysteine. These inhibitors modify the cell growth depending on the cystine concentration in the medium. They inhibit the growth when cystine concentration is physiological. An excessive concentration of cystine is in itself growth inhibitory, but this inhibitory action is antagonized by glutamate or homocysteate.