Label-free single-cell protein quantification using a drop-based mix-and-read system.
Label-free single-cell protein quantification using a drop-based mix-and-read system.
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使用基于液滴的混合读取系统进行无标记单细胞蛋白质定量
DOI：
10.1038/srep12756
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发表时间：
2015-08-03
影响因子：
4.6
通讯作者：
Weitz DA
中科院分区：
文献类型：
--
作者：
Abbaspourrad A;Zhang H;Tao Y;Cui N;Asahara H;Zhou Y;Yue D;Koehler SA;Ung LW;Heyman J;Ren Y;Ziblat R;Chong S;Weitz DA
由于检测一个细胞中存在的微量蛋白质的技术困难，单细胞的定量蛋白质分析很少实现。我们开发了一种混合读取测定法，用于单细胞的滴式无标记蛋白质分析。这种高通量方法量化蛋白质的绝对量，而不是相对量，并且不涉及抗体标记或质谱分析。
Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.